Plasmids and DNA libraries
Training programme in preservation and characterisation of bacteria
Our scientists and technicians have a wealth of experience in the preservation and characterisation of bacteria, and are committed to passing on that knowledge. BCCM/LMG will follow up on its successful participation in the Training and Outreach Programme of the past EU-FP7 EMbaRC project (www.embarc.eu) by organising a training programme once a year. This programme will combine interactive oral lectures with hands-on sessions during which participants can benefit from expert advice, advanced equipment and access to taxonomic databases.
Depending on the personal interests and research needs of the applying participants, one or both of the two themes below can be included in the training programme.
Bacterial collection management
- Acquisition and distribution of bacterial cultures: legal aspects and standard practices
- Quality control of bacteriological material
- Long-time preservation of bacterial cultures through lyophilisation
Taxonomy, identification and typing of bacteria
- General principles of bacterial taxonomy
- State-of-the-art overview of methods for bacterial identification and typing
- Processing and interpretation of taxonomic data (e.g. DNA sequences, DNA fingerprints, MALDI-TOF MS spectra, FAME profiles, DNA-DNA hybridisation data)
- Case study: identification and/or typing of a limited number of isolates brought by the participants (after mutual agreement on the study outline with BCCM/LMG)
Location: BCCM/LMG Bacteria Collection & Laboratory of Microbiology, Ghent University, K.L. Ledeganckstraat 35, B-9000 Gent, BELGIUM
Period: Once a year
Duration: app. two weeks
Number of participants: min. 2 - max. 5 participants/training programme
The registration fee includes access to the training programme, scientific documentation, a proof of participation and lunches (as scheduled in the programme). Accommodation and food not included.
To express your interest in attending this training programme, please contact BCCM/LMG.
General training in mycology can be offered :
- 'Fungi of the environment' (Contact Dr. D. Stubbe firstname.lastname@example.org)
General training in mycology can be offered under different objectives:
- Isolation, culture and preservation methods
- Conventional and molecular identification
Representative strains of the major taxonomic groups of fungi have been selected for teaching purposes and are available at a reduced price (see pricelist).
The CEnter of Study on Arbuscular Mycorrhizal Monoxenics (CESAMM) and the Glomeromycota IN vitro COllection (MUCL/GINCO) have made the in vitro cultivation of AM fungi their primary research tool to study these fungi and their relationships with their host plants. Since 2005, CESAMM and GINCO organize an international training to researchers, technicians and industrials on the techniques to culture AM fungi under in vitro conditions. This training is organized each year in late May.
Plasmids and DNA libraries
General training in DNA handling techniques:
- Plasmid DNA isolation and transformation
- Restriction enzyme analysis and gel electrophoresis
- PCR and cloning techniques
- Plasmid DNA preservation methods
Fee: case by case
Plasmid collection management:
- Acquisition and distribution of plasmids: legal aspects and standard practices
- Quality management system / quality control tests
- Data processing
- Long-time preservation of (plasmid-carrying) bacterial cultures and isolated DNA
- Biosafety and biosecurity matters
- Autonomous research
- International cooperation
Duration: 1 day (presentation + guided tour)