GREAT AT SMALL THINGS

FAQ

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  • Are certificates provided for strains that were ordered?

    The package with the strains will include a delivery note with the following information: strain number, species name, conformity of viability, purity, identity tests, recommended culture medium and incubation temperature as well as the country of origin and biological origin. In case you need further certification, please contact the collection.

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  • How can I obtain material from a patent deposit?

    The BCCM collections are approved as International Depository Authorities and operate under the Budapest Treaty to make patent materials available.. Requests for patented material should be joined by written approval of the relevant patent office or the depositor.

  • How can I pay?

    Since the collections belong to different host institutions, they are required to follow the rules and conditions imposed by each institutions finance administration.

     

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                BCCM/LMBP: Our alternatives for payment by credit card are by bank transfer, with or without     Purchase Order, or by check. Our invoices indicate all necessary information for payment by            bank transfer.

                BCCM/ITM: We accept payment by credit card and by bank transfer, all necessary          information can be found on the invoice

                BCCM/IHEM: We accept payment by credit card and by bank transfer, all necessary        information can be found on the invoice.

     

                To be checked by other collections.

                BCCM/MUCL: Due to rules imposed by the host University we cannot accept payment by            credit card. The only accepted payment mode is by bank transfer, with or without Purchase    Order number. Our invoices indicate all necessary information for payment by bank transfer.

    BCCM/LMG : Payment by credit card is temporarily not possible. As from April it should be possible again. Payment can be made by bank transfer or by check. Our invoices indicate all necessary information for payment by bank transfer.

                BCCM/ULC : Due to rules imposed by the host University we cannot accept payment by credit card. The only accepted payment mode is by bank transfer, with or without Purchase    Order number. Our invoices indicate all necessary information for payment by bank transfer.

                BCCM/DCG:

     

  • How many bacterial cells are there in an ampoule of lyophilised material?

    On average, our ampoules with freeze dried bacteria contain 1010 living cells/ml. One ampoule contains 0.1 ml (100 µL).

  • How should I handle arrayed sets?

    Instructions are shown in the following video: link https://www.youtube.com/watch?v=UypUrivSnP4

  • How should ampoules with lyophilised material be stored?

    Freeze dried cultures stored in their intact glass ampoules at 4°C to 6°C in the dark; generally remain viable for many years after production.

  • How should cultures be recovered from ampoulles containing lyophilised material?

    The ampoule should be scored by a sharp file just above the cotton plug. Then, the outside should be disinfected by a lightly alcohol-dampened pad. A protective pad should be wrapped around the ampoule, which should be broken by applying pressure to the opposite side of the score. 0.3 to 0.5 ml of a sterile liquid (water or broth) should be added using a sterile Pasteur pipette. Allow the lyophilised material to rehydrate for at least 30 min before pipetting the liquid onto the surface of a suitable growth medium. Incubate at an appropriate temperature. The remains of the ampoule should be autoclaved before disposal.

  • How should living cultures of fungi be stored, if not used immediately?

    Living cultures on agar slants can be stored at 4°C for a short period only.

    It is advised to recover the culture immediately on receipt by subculturing onto fresh medium before use or storage. For longer storage you can either prepare a cryovial for storage at -80°C or lower containing the biological material and 10% glycerol or lyophilise the biological material with suitable protectants such as trehalose.

  • How should plasmids be stored, if not used immediately?

    Plasmid-carrying E. coli culture can be stored at 4°C for a short period.

    It is advised to recover the culture immediately on receipt by subculturing into liquid or on solid medium before use or storage.

  • How to deposit biological material in a BCCM collection?

    How to provide information on the biological material?

    It is recommended to contact the BCCM collection in which you want to deposit your material to confirm that your material is within the collection's scope and technical capability. To facilitate the information-based evaluation of the deposit it is recommended to complete and submit the accession form (http://bccm.belspo.be/docs/deposit-forms) before sending the biological material. However, you may send the material together with the accession form if you strongly assume that the deposit is within the scope of the collection.

     

    How to provide the biological material?

     

    Cultures should be fresh when leaving your lab and be packaged to prevent breakage.

    Petri dishes as primary recipients should be avoided if possible. Screw cap tubes are preferred. Glass material may be used, but obviously bears a greater risk of breakage to be counteracted by appropriate packaging. For plasmids, isolated DNA is preferred.

    Organisms of Risk Group 2 and 3 require packaging according to the UN Model Regulations for correct packaging and shipping of Dangerous Goods (include link). This implicates that a special type of packaging must be used and that the parcel must be transported by a recognized courier.

    It is the sender's responsibility to comply to the national regulations for the export of biological material and include any required export permit and certificates.

    Avoid excessive exposure to heat and cold as well as long transport delays as much as possible.

     

  • How to find biological material and the associated information?

    Most strains and biological materials are listed with their associated information in the online catalogues found at (achter catalogueshttp://bccm.belspo.be/catalogues). Exceptions are materials that are deposited confidentially and materials that are under research. Additional information may be obtained from the literature references in the individual strain entries. BCCM materials are cross-referenced to materials of more than 60 other collections via http://www.straininfo.net/.

    You can type a strain number or species name in the search field and immediately see the equivalent number in the other collections.

  • How to make an (first) order?

    If you can’t decide for a particular strain, please contact the collection mentioning the species and/or purpose you require the biological material for. The collection will try to help you selecting the appropriate biological material.

    • Read and send the approval of the MTA by an authorised person.
    • Provide a proof of licence to handle the material in your laboratory in case of risk class 3 organisms.
    • Provide any import permit if required by your country.
    • First orders should be sent to our attention on your company’s letterhead paper, signed by the authorised person.
    • Subsequent orders can be made online via the shopping card or by email, fax or letter.

     

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  • In which form will the biological material be delivered?

    The form of supply is depending on the type of biological material that is requested and from which collection it is requested. The collection-specific forms of supply are shown on each collections catalogue search page.

     

  • Is there a charge for depositing biological material in a BCCM collection?

    Deposit of biological material in a public collection of the BCCM is free of charges to the depositor. Charges for the deposit of biological material for patent purposes or as a safe deposit are given on our website.

     

    http://bccm.belspo.be/services/deposit#public-deposit

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    Interdum et malesuada fames ac ante ipsum primis in faucibus. Quisque erat justo, bibendum quis porttitor at, rutrum quis felis. Vestibulum laoreet ligula arcu, eget accumsan velit accumsan id. Nam imperdiet, elit imperdiet imperdiet interdum, nunc sapien dapibus lacus, vel eleifend tortor libero id felis. Phasellus vehicula id mi nec convallis. Integer dapibus nibh velit, non congue est sagittis placerat. Morbi vestibulum mauris sit amet nisi aliquet ullamcorper. Nulla euismod tortor tortor, eget pulvinar est rutrum at.

  • Wath is the delivery time (lead time) of the biological material?

    The delivery time depends on the type of the biological material and the specific procedures in the different collections.

    After receiving all information necessary to approve the order (including the approval of the MTA):

     

    BCCM/LMBP: shipment of biological material is foreseen [LR1] within with one week (plasmids with core status) or within three weeks (plasmids with non-core status).

                BCCM/MUCL: shipment of biological material is foreseen within two weeks. Different       arrangements are possible in consultation with the collection in particular for large       batches of strains or for specific delivery dates.

                BCCM/ITM: within two weeks for freeze-dried material or DNA, or within 1-2 months in case          fresh cultures are requested.

                BCCM/IHEM: freeze dried material in stock is sent within one week, in case the material is not      in stock or a fresh culture is requested, the client is contacted, but the order is generally sent           out within 3 weeks.

    BCCM/LMG : shipment of freeze dried material is foreseen within one week, shipment of a fresh culture within two weeks, shipment of DNA within 3 – 5 weeks

                BCCM/ULC : within 15 working days, except for specific cases after discussion with the client.

                BCCM/DCG :

     

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  • What are the key specificities of safe and patent deposits?

    Safe deposits are confidential during the entire period of the contract. Distribution is limited to the depositor and depositor-authorised third parties. The depositor decides about the fate of the material at the end of the contract.

    Patent deposits serve the purpose to make patents reproducible. Patent deposits are restricted to be accessed by the depositor, the relevant patent office and authorised third parties.

  • What is a typestrain?

    A type strain is designated in the description of a new species (International Code of Nomenclature for algae, fungi and plants: http://www.iapt-taxon.org/nomen/main.php and International Code Nomenclature for Bacteria: LINK). It is the single reference strain of a species to which other strains need to be compared for valid inclusion in that species (i.e. identification). The type strain is the validated nomenclatural representative of a species, but is not necessarily the most typical representative of its species. If a BCCM collection strain is a type strain, this is specifically indicated in the catalogue field ‘status’ (http://bccm.belspo.be/catalogues).

  • What is the VAT number of the different institutes?

    Ugent (Universiteit gent) for BCCM/LMG, BCCM/LMBP, BCCM/DCG

         BE 0248015142

       UCL (Université Catholique Louvain) for BCCM/MUCL    

         BE 0419052272

       ITG ( Instituut Tropische Geneeskunde) for BCCM/ITM

         BE 0410 057 701

    WIV/ISP (Instituut Volksgezondheid) for   BCCM/IHEM         

         BE 0254.014.195

     ULG (Université Liège) for  BCCM/ULC         

         BE 0325777171

              

  • What is the price of the biological materials

    Pricelists specific to each BCCM collection are available from the webpage:

    http://bccm.belspo.be/pricelists.

  • When can an invoice be expected?

    Since the collections belong to different host institutions, they are required to follow the rules      and conditions imposed by each institutions finance administration.

    BCCM/LMBP: invoices are clustered and made every two months. This means invoices are sent at the end of February, April, June, August, October and December.

                BCCM/MUCL: In general invoices are sent a few days after the shipment of the biological            material. In some cases, the invoice is already included in the shipment.

                BCCM/ITM : Pro-forma invoice for new foreign customers upon first order. Regular invoices for   known customers are made weekly.

    BCCM/IHEM: invoices are sent at the end of each month. Only for new, foreign customers, the    first order is invoiced immediately and shipment will be done after payment.
                BCCM/LMG : In general invoices are sent within 2 weeks after the shipment of the biological material.

                BCCM/ULC : In general, invoices are sent a few days after shipment of the biological material.

                BCCM/DCG :

     

     

  • Which bank details can be used in order to add BCCM collections to your vendor database?

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    BCCM/LMBP: (Financiële identificatiegegevens.pdf)

                BCCM/MUCL: All necessary information is mentioned on every invoice. (harmonisation by           including pdf as well)

                BCCM/ITM   : (Account Information ITM.pdf)

                BCCM/IHEM: (BCCM-Payment form.pdf)

                BCCM/LMG : Our bank details are mentioned on every invoice. (Financial identification.pdf)

                BCCM/ULC :

                BCCM/DCG :

  • Which growth conditions are recomended for a particular strain?

    The recommended growth conditions are shown in the online catalogues and on the delivery note that accompanies the delivered strains. These conditions should be used for the initial subculture from the BCCM provided material. Media abbreviations refer to common microbiological growth media. Their composition will be made available on request.