Following the 2009-2010 call of the Hercules Foundation for medium-scale research infrastructure1, BCCM/LMBP became a partner in a collaboration of scientists of the Ghent University and the VIB who are working in the domain of life sciences and have already extensive experience with the use of RNA interference (RNAi).
RNAi has become an essential tool for functional genomics. It permits the selective knock-down of specific proteins in cells to assess their functional role. RNAi serves to discover proteins involved in a particular cell function or signal transduction pathway. It is increasingly used to discover and validate new targets and biomarkers, and to elucidate disease mechanisms. Moreover, it is being developed for in vivo experimental use and even for therapeutic use.
BCCM/LMBP offers easy access to both individual bacterial cultures and isolated plasmid DNA of the genome-wide SIGMA MISSION TRC1 and part of the TRC1.5 human and mouse libraries, consisting of over 150.000 pre-cloned shRNA vectors targeting more than 15.000 human and 15.000 mouse genes.
Unlimited access is provided to all researchers of Ghent University and VIB.
Other users have access to a limited number of clones per RNAi library per year in the context of a collaboration with either UGent or VIB.
More info: bccm.lmbp@irc.UGent.be
Template structure of each shRNAi clone
- for VIB researchers: consult the VIB intranet under the topics 'VIB Technologies > 3. Protein analysis > 9. Human and mouse gene shRNA plasmids'
- for IRC researchers: consult the IRC intranet under the topics 'Research > Core Facilities > BCCM Plasmid Collection', after logging in
- for Ghent University researchers: click here
- for researchers collaborating with UGent or VIB: click here.
1 Set up in 2007 by the Flemish Government as a structural funding instrument for investments in research infrastructure.