GREAT AT SMALL THINGS

0

LMBP plasmid details

Last data update: 10 July 2020 04:20 CEST

Plasmid name: pYFP-C-GW (LMBP 8362)

Add to cart

New search

Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p8362.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: B gene of the control of cell death locus of the Escherichia coli F plasmid (ccdB, lethal gene)
Aequorea victoria green fluorescent protein DNA (GFP); enhanced yellow-green fluorescent variant (EYFP), C-terminal
Promoter: Human cytomegalovirus immediate early promoter (CMV-IE) and enhancer
Escherichia coli lac operon promoter; mutant (lacUV5)
Escherichia coli β-lactamase promoter (amp)
Simian virus 40 early promoter (SV40 early)
Ribosome
binding site:
Ribosome binding site (RBS) of the Escherichia coli ampicillin resistance gene (amp)
Terminator: Herpes simplex virus (HSV) thymidine kinase polyadenylation signal (TK polyA)
Simian virus 40 polyadenylation signal (SV40 polyA)
Selection marker: Neomycin (neo; G418; kanamycin (kan))
Chloramphenicol (cam)
Replicon: Simian virus 40 bidirectional origin (SV40)
Escherichia coli plasmid pMB1 origin
Phage f1 origin
Host range: Escherichia coli; use a ccdB-resistant strain for propagation
Mammalian cells; SV40 permissive cells
Parental clone: pEYFP-C1
Further information: This is a Gateway destination vector meant for C-terminal fusion of the EYFP reporter gene.
EMBL Accession number: -
Latest sequence update: 03/06/2013
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr S. Goethals(1) and Prof. Dr V. Timmerman(1). It was constructed by Dr L. de Almeida Souza(2).
(1) Department of Molecular Genetics, VIB, Antwerp, Belgium
(2) Neurobiology Division, MRC Laboratory of Molecular Biology, Cambridge, UK
Plasmid reference: -
Restricted distribution: - BCCM MTA
- The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC.
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12xB DB3.1 (LMBP 04098)
Host reference: -
Related host reference: Bernard et al., J. Mol. Biol. 226 (1992), 735-745 [PMID: 1324324]
Cultivation medium: LB-Lennox + chloramphenicol (34 μg/ml) + kanamycin (50 μg/ml)*
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: *: selection of transformants on chloramphenicol; subsequent cultivation of a single colony in liquid medium with kanamycin.
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

New search