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LMBP plasmid details

Last data update: 05 March 2021 04:23 CET

Plasmid name: pSV23E6Hy3 (LMBP 1684)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p1684.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Mouse anti-hPLAP monoclonal antibody E6(Hy3,κ) half genomic gene; mouse::human chimeric heavy chain (E6Hy3)
Promoter: Simian virus 40 early promoter (SV40 early)
Ribosome
binding site:
-
Terminator: Simian virus 40 polyadenylation signal (SV40 polyA)
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Simian virus 40 bidirectional origin (SV40)
Host range: Escherichia coli
Mammalian cells; SV40 permissive cells
Parental clone: pSV23; pATE6Hy3
Further information: This plasmid contains a half-genomic insert encoding the complete mouse::human chimeric heavy chain of the anti-hPLAP antibody E6(Hy3,κ) clockwise orientated relative to the SV40 early promoter. The mouse part of the cloned gene consists of cDNA; the human part consists of genomic DNA.
The plasmid doesn't contain the SV40 small t-antigen splicing signal.
Designed for transient expression in COS cells or constitutive expression in CHO cells (cotransfection with DHFR plasmids).
The HindIII-SalI fragment of pATE6Hy3 is cloned in the HindIII-SalI site of pSV23.
Other name of the plasmid is pSV23Hy3.
EMBL Accession number: -
Latest sequence update: 04/04/1989
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by V. Feys(1) and Prof. Dr W. Fiers(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Related plasmid reference: De Sutter et al., Gene 113 (1992), 223-230 [PMID: 1572543]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12xB HB101
Host reference: Boyer and Roulland-Dussoix, J. Mol. Biol. 41 (1969), 459-472 [PMID: 4896022]
Related host reference: Sambrook et al. (eds), Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, NY (1989) [ISSN/ISBN: 0879693096]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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