LMBP plasmid details

Last data update: 24 November 2020 04:18 CET

Plasmid name: pPSDalpha-FLAG-V5-SAG1 (LMBP 10236)

Add to cart

New search

Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: -
analysis results


Cloned DNA: Saccharomyces cerevisiae α-mating factor 1 gene (MFα1, GeneID 855914); prepro secretion signal sequence (ppMF)
FLAG epitope tag; N-terminal
V5 epitope; C-terminal
Saccharomyces cerevisiae alpha-agglutinin cDNA (SAG1, AG(ALPHA)1, GeneID 853460); C-terminal fragment
Promoter: Pichia pastoris alcohol oxidase 1 promoter (AOX1)
Saccharomyces cerevisiae translation elongation factor 1α promoter (TEF1α)
Synthetic prokaryotic EM7 promoter
binding site:
Terminator: Pichia pastoris alcohol oxidase 1 terminator (AOX1)
Saccharomyces cerevisiae iso-1-cytochrome C terminator (CYC1)
Selection marker: Zeocin (zeo)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Pichia pastoris; integrative
Parental clone: pPSDalpha-FLAG-stuffer-V5-SAG1
Further information: The plasmid was constructed by removing the stuffer fragment from pPSDalpha-FLAG-stuffer-V5-SAG1.
The plasmid is intended for surface display of proteins in P. pastoris using the S. cerevisiae SAG1 protein.
Protein expression is controlled by the methanol-induced AOX1 promoter.
The protein of interest is cloned behind a preproMF secretion signal and the N-terminal FLAG tag, and in front of the V5 tag and the SAG1 protein, using SfiI-PacI restriction enzymes.
Integration into the AOX1 promoter can be obtained via linearisation with MssI or PmeI.
Other name of the plasmid is pPSDzeoSfiIPacI-FLAGV5-AOX1-EV.
EMBL Accession number: -
Latest sequence update: 10/08/2017
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr N. Callewaert(1) (2).
(1) VIB-UGent Center for Medical Biotechnology, VIB, Ghent, Belgium
(2) Department of Biochemistry and Microbiology, Ghent University, Ghent, Belgium
Plasmid reference: PhD thesis Morgane Boone (2017)
Related plasmid reference: Ryckaert et al., J. Biotechnol. 145 (2010), 93-98 [PMID: 19861136] [DOI: 10.1016/j.jbiotec.2009.10.010]
Restricted distribution: - BCCM MTA
- The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC.
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 TOP10
Host reference: Invitrogen Instruction Manual
Related host reference: Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051]
Cultivation medium: LB-Lennox + zeocin (25 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

New search