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LMBP plasmid details

Last data update: 24 November 2020 04:18 CET

Plasmid name: pPSALUC1 (LMBP 2927)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: not available
Sequence
analysis results
Genecorner:

-

Cloned DNA: Photinus pyralis (firefly) luciferase gene (LUC); 5' fragment
Promoter: Staphlylococcus aureus protein A promoter (psa)
Ribosome
binding site:
Ribosome binding site (RBS) of the Staphlylococcus aureus protein A (spa) gene
Terminator: -
Selection marker: Ampicillin (amp)
Chloramphenicol (cam)
Replicon: Escherichia coli plasmid pMB1 origin
Staphylococcal plasmid pC194 origin
Host range: Escherichia coli
Staphylococcus aureus
Parental clone: pMaLUC2; pPSA2
Further information: -
EMBL Accession number: -
Latest sequence update: 21/02/2002
Sequence detail:
Sequence ot the start of the luciferase gene:

         S. aureus protein A   -->|--> luciferase
5' GACTTTACTAGTACATACAGGGGGTATTAAC.ATG.GAA.GAC.GCC.AAA. 3'
           * *   -----------         * *
         SpeI       S.D.           Met Glu Asp Ala Lys

* : mutated nucleotide
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Y. Wei(1) (2), Dr L. Steidler(1) (2) and Prof. Dr E. Remaut(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: Steidler et al., Appl. Environ. Microbiol. 62 (1996), 2356-2359 [PMID: 8779573]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml) + chloramphenicol (34 μg/ml)*
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: *: selection of transformants on chloramphenicol; subsequent cultivation of a single colony in liquid medium with ampicillin.
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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