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LMBP plasmid details

Last data update: 24 November 2020 04:18 CET

Plasmid name: pPS3 (LMBP 9598)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p9598.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Saccharomyces cerevisiae drug-responsive transcription factor 1 cDNA (PDR1)
Myc epitope; N-terminal
Promoter: Saccharomyces cerevisiae drug-responsive transcription factor 1promoter (PDR1)
Phage T3 promoter
Phage T7 gene 10 promoter (T7g10)
Ribosome
binding site:
-
Terminator: Saccharomyces cerevisiae drug-responsive transcription factor 1 terminator (PDR1)
Selection marker: Ampicillin (amp)
Saccharomyces cerevisiae LEU2; auxotrophic
Replicon: Escherichia coli plasmid pMB1 origin
Saccharomyces cerevisiae centromeric region 6 (CEN6)/ S. cerevisiae autonomously replicating sequence ARSH4
Phage f1 origin
Host range: Escherichia coli
Saccharomyces cerevisiae; leu2(-)
Parental clone: pRS315
Further information: The plasmid was constructed by cloning the Myc-tagged S. cerevisiae PDR1 coding sequence, under the control of its own promoter and enhancer, into the pRS315 vector.
The nucleotide sequence of the S. cerevisiae PDR1 cDNA, PDR1 promoter and PDR1 terminator corresponds with the EMBL Nucleotide Sequence Database accession number J03487.1.
The nucleotide sequence of the pRS315 corresponds with the EMBL Nucleotide Sequence Database accession number U03441.1.
EMBL Accession number: J03487.1, view at EMBL, GenBank, DDBJ
U03441.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 05/08/2015
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr S. Moye-Rowley (1). It was constructed by P. Shahi (1).
(1) Laboratory of Molecular and cellular biology of eukaryotic stress resistance, Department of Molecular Physiology and Biophysics, Carver College of Medicine, University of Iowa, Iowa City, USA
Plasmid reference: -
Related plasmid reference: Khakhina et al., Eukaryotic Cell 14 (2015), 442-453 [PMID: 25724885] [DOI: 10.1128/EC.00021-15]
Restricted distribution: - BCCM MTA
- The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC.
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 DH5α
Host reference: Focus 8 (1986), 9
Related host reference: Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051]
Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187]
Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791]
Rodriguez-Quinones et al., Focus 15 (1993), 110-112
Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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