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LMBP plasmid details

Last data update: 24 November 2020 04:18 CET

Plasmid name: pPR395Ec (LMBP 607)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: not available
Sequence
analysis results
Genecorner:

-

Cloned DNA: Phage λ cI repressor gene (cI, lambdap51, GeneID 2703538); N-terminal fragment
Promoter: Phage λ major rightward promoter (λ PR)
Phage λ promoter for repressor maintenance (λ PRM)
Ribosome
binding site:
-
Terminator: -
Selection marker: Ampicillin (amp)
Tetracycline (tet)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli; use strains with a cI function, cIts for PL controlled expression
Parental clone: pBR322
Further information: This plasmid contains a λ RsaI fragment, containing the first 104 codons of the phage λ cI repressor gene, cloned into the EcoRI site of pBR322 via EcoRI linkers.
The orientation of the cloned EcoRI fragment is not known.
EMBL Accession number: -
Latest sequence update: 18/03/1992
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: EcoRI, HindII, HindIII and RsaI. The pattern does not fit the expected plasmid length and fragment sizes deduced from the nucleotide sequence file that can be compiled according to the available data on the construction: the plasmid length seems to be more than 6000 bp. The presence of a 400 bp EcoRI fragment, corresponding to the length of the cI insert, could be confirmed. According to the depositor, an unknown fragment might have been fortuitously picked up during construction, without any influence on the insert and the ampicillin and tetracycline resistance markers.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr E. Remaut(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 r-m+ (λ)
Host reference: -
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml) + tetracycline (10 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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