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LMBP plasmid details

Last data update: 24 November 2020 04:18 CET

Plasmid name: pPLi342 (LMBP 1221)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: ppli342.gb (View with Genome Compiler)
ppli342.pdf
Sequence
analysis results
Genecorner:

-

Cloned DNA: Phage λ cI temperature-sensitive repressor gene (cIts, cI857)
Promoter: Phage λ major leftward promoter (λ PL)
Phage λ major rightward promoter (λ PR)
Phage λ promoter for repressor maintenance (λ PRM)
Ribosome
binding site:
-
Terminator: -
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Parental clone: pAT153cI857; pPLi34
Further information: This plasmid contains the cI857 temperature-sensitive repressor gene.
Designed for λ PL promoter-regulated expression of fragments containing a ribosome binding site functional in Escherichia coli.
The orientation of the plasmid origin is clockwise (inverted relative to pBR, pPLc, pPLa plasmids).
The plasmid displays temperature-dependent runaway replication.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727572.1.
EMBL Accession number: LT727572.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 25/03/2011
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: EcoRI, NdeI/NsiI and XbaI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr E. Remaut(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 28°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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