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LMBP plasmid details

Last data update: 24 November 2020 04:18 CET

Plasmid name: pPLcmuMHTNF4 (LMBP 2530)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p2530.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Mouse tumor necrosis factor (Tnf, TNF); fragment
Human tumor necrosis factor (TNF); fragment
Promoter: Phage λ major leftward promoter (λ PL)
Ribosome
binding site:
Ribosome binding site (RBS) of the phage Mu ner protein gene
Terminator: -
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli; use strains with a cI function, cIts for PL controlled expression
Parental clone: pPLcmuHTNF1; pATTrpMTNF
Further information: This plasmid was constructed as follows : the NcoI-BanI fragment of pATTrpMTNF (containing the first part of mature mouse TNF) was inserted into the NcoI-BanI sites of pPLcmuHTNF1.
pPLcmuMHTNF4 is designed for a PL-controlled expression of intracellular mature human-mouse hybrid TNF.
Restriction analysis revealed that the plasmid is approx. 1600 bp shorter as described. Supposedly the plasmid is derived from a pPLc245HTNF-plasmid and not from pPLcmuHTNF1. The nucleotide sequence file has not been adapted.
Other names of the plasmid are MH4 and pPLc245-MH4.
EMBL Accession number: M11731, view at EMBL, GenBank, DDBJ
X01394, view at EMBL, GenBank, DDBJ
Latest sequence update: 17/01/1992
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr E. Remaut(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: Tavernier et al., J. Mol. Biol. 211 (1990), 493-501 [PMID: 1689779]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 DH1(λ)
Host reference: Bachmann, in 'Escherichia coli and Salmonella: Cellular and Molecular Biology', American Society for Microbiology, Washington DC (1987), 1190-1219
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 28°C
Biosafety level: L1
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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