Last data update: 24 November 2020 04:18 CET
Plasmid name: pPLcmuMHTNF15 (LMBP 2571)
|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner non-core plasmid|
|Cloned DNA:||Mouse tumor necrosis factor (Tnf, TNF); fragment
Human tumor necrosis factor (TNF); fragment
|Promoter:||Phage λ major leftward promoter (λ PL)|
|Ribosome binding site (RBS) of the phage Mu ner protein gene|
|Selection marker:||Ampicillin (amp)|
|Replicon:||Escherichia coli plasmid pMB1 origin|
|Host range:||Escherichia coli; use strains with a cI function, cIts for PL controlled expression|
|Parental clone:||pPLcmuMHTNF14; pPLcmuHTNF1|
|Further information:||pPLcmuMHTNF15 is designed for a PL-controlled expression of intracellular mature human-mouse hybrid TNF.
To construct this plasmid three fragments were ligated : the large SalI-EcoRI fragment (containing AMP, the ori
and the PL promoter), the EcoRI-HindII fragment (containing hybrid TNF), both from pPLcmuMHTNF14, and the HindII-SalI
fragment (part of human TNF) from pPLcmuHTNF1.
Other name of the plasmid is pPLcmuMHY3.
|EMBL Accession number:||M11731, view at EMBL, GenBank, DDBJ
X01394, view at EMBL, GenBank, DDBJ
|Latest sequence update:||17/01/1992|
|Authenticity test:||The plasmid still needs to be subjected to the authenticity test.|
|History of deposit:||This plasmid was deposited by Prof. Dr E. Remaut(1) (2).
It was constructed by Yves Desauw.
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 MC1061(λ)|
|Host reference:||Mertens et al., Gene 164 (1995), 9-15 [PMID: 7590329]
|Related host reference:||Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
|Cultivation medium:||LB-Lennox + ampicillin (100 μg/ml)|
|Other culture collection numbers:||-|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.