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LMBP plasmid details

Last data update: 24 November 2020 04:18 CET

Plasmid name: pPLcmuMHTNF15 (LMBP 2571)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p2571.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Mouse tumor necrosis factor (Tnf, TNF); fragment
Human tumor necrosis factor (TNF); fragment
Promoter: Phage λ major leftward promoter (λ PL)
Ribosome
binding site:
Ribosome binding site (RBS) of the phage Mu ner protein gene
Terminator: -
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli; use strains with a cI function, cIts for PL controlled expression
Parental clone: pPLcmuMHTNF14; pPLcmuHTNF1
Further information: pPLcmuMHTNF15 is designed for a PL-controlled expression of intracellular mature human-mouse hybrid TNF.
To construct this plasmid three fragments were ligated : the large SalI-EcoRI fragment (containing AMP, the ori
and the PL promoter), the EcoRI-HindII fragment (containing hybrid TNF), both from pPLcmuMHTNF14, and the HindII-SalI
fragment (part of human TNF) from pPLcmuHTNF1.
Other name of the plasmid is pPLcmuMHY3.
EMBL Accession number: M11731, view at EMBL, GenBank, DDBJ
X01394, view at EMBL, GenBank, DDBJ
Latest sequence update: 17/01/1992
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr E. Remaut(1) (2).
It was constructed by Yves Desauw.
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061(λ)
Host reference: Mertens et al., Gene 164 (1995), 9-15 [PMID: 7590329]
Related host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 28°C
Biosafety level: L1
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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