Last data update: 24 November 2020 04:18 CET
Plasmid name: pPLcZCYA1 (LMBP 488)
|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner non-core plasmid|
|Cloned DNA:||Phage MS2 polymerase gene; fragment
Escherichia coli lac Z gene (lacZ)
Escherichia coli lac Y gene (lacY)
Escherichia coli lac A gene (lacA)
|Promoter:||Phage λ major leftward promoter (λ PL)|
|Ribosome binding site (RBS) of the phage MS2 polymerase gene|
|Selection marker:||Ampicillin (amp)|
|Replicon:||Escherichia coli plasmid pMB1 origin|
|Host range:||Escherichia coli; use strains with a cI function, cIts for PL controlled expression|
|Parental clone:||pPLcZCYA0; pPLc24|
|Further information:||This plasmid is similar to pPLcZCY1, but it contains the complete lacY gene as well as the lacA gene.
This plasmid contains the coding sequence for a fusion protein between the first 99 amino acids of MS2 polymerase and β-galactosidase.
Expression of a carboxy terminal lacZ and an amino terminal MS2 polymerase (99 amino acids) fused protein is possible via the BamHI site (phase for both fusions: GAT).
The natural EcoRI site at the end of lacZ gene was removed by mutation.
Other name of the plasmid is pPLc236LacPol2.
|EMBL Accession number:||-|
|Latest sequence update:||31/01/1989|
Nucleotide sequence at the junction of the MS2 polymerase gene and the lacZ gene: 98 9 10 5' TGG.GAT.CCC.GTC.GTT 3' * BamHI ^ *: 98th codon of the MS2 polymerase gene. ^: 9th codon of the lacZ gene. Punctuation indicates the reading frame.
|Authenticity test:||The plasmid still needs to be subjected to the authenticity test.|
|History of deposit:||This plasmid was deposited by Prof. Dr E. Remaut(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 DS410λcIts (BA)|
|Cultivation medium:||LB-Lennox + ampicillin (100 μg/ml)|
|Other culture collection numbers:||-|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.