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LMBP plasmid details

Last data update: 24 November 2020 04:18 CET

Plasmid name: pPLcZCYA1 (LMBP 488)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p488.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Phage MS2 polymerase gene; fragment
Escherichia coli lac Z gene (lacZ)
Escherichia coli lac Y gene (lacY)
Escherichia coli lac A gene (lacA)
Promoter: Phage λ major leftward promoter (λ PL)
Ribosome
binding site:
Ribosome binding site (RBS) of the phage MS2 polymerase gene
Terminator: -
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli; use strains with a cI function, cIts for PL controlled expression
Parental clone: pPLcZCYA0; pPLc24
Further information: This plasmid is similar to pPLcZCY1, but it contains the complete lacY gene as well as the lacA gene.
This plasmid contains the coding sequence for a fusion protein between the first 99 amino acids of MS2 polymerase and β-galactosidase.
Expression of a carboxy terminal lacZ and an amino terminal MS2 polymerase (99 amino acids) fused protein is possible via the BamHI site (phase for both fusions: GAT).
The natural EcoRI site at the end of lacZ gene was removed by mutation.
Other name of the plasmid is pPLc236LacPol2.
EMBL Accession number: -
Latest sequence update: 31/01/1989
Sequence detail:
Nucleotide sequence at the junction of the MS2 polymerase gene and the lacZ gene:

    98          9   10
5' TGG.GAT.CCC.GTC.GTT 3'
   * BamHI     ^

*: 98th codon of the MS2 polymerase gene.
^: 9th codon of the lacZ gene.
Punctuation indicates the reading frame.
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr E. Remaut(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 DS410λcIts (BA)
Host reference: -
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 28°C
Biosafety level: L1
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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