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LMBP plasmid details

Last data update: 24 November 2020 04:18 CET

Plasmid name: pPLcZCYA0 (LMBP 489)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p489.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Escherichia coli lac Z gene (lacZ); starting at the 9th codon
Escherichia coli lac Y gene (lacY)
Escherichia coli lac A gene (lacA)
Promoter: Phage λ major leftward promoter (λ PL)
Ribosome
binding site:
-
Terminator: -
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli; use strains with a cI function, cIts for PL controlled expression, the detection host must also be lacZ(-).
Parental clone: pPLc236; pMC1403
Further information: Used for detection of prokaryotic translation-initiation domains after insertion into either BamHI, EcoNI, EcoRI or SmaI to make fusion proteins with β-galactosidase.
The natural EcoRI site at the end of the lacZ gene was removed by mutation.
Other name of the plasmid is pPLc236Lac.
EMBL Accession number: -
Latest sequence update: 30/01/1989
Sequence detail:
Nucleotide sequence of the polylinker downstream from the PL promoter:

5' GA.ATT.CCC.GGG.GAT.CCC.GTC ----- coding region of lacZ ---- 3'
   EcoRI  SmaI  BamHI     *

*: 9th codon of the lacZ gene.
Punctuation indicates reading frame.
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr E. Remaut(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061[pRK248cIts]
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Helper plasmid: pRK248cIts
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 28°C
Biosafety level: L1
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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