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LMBP plasmid details

Last data update: 24 November 2020 04:18 CET

Plasmid name: pPLcZCY1 (LMBP 532)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: pplczcy1.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Phage MS2 polymerase gene; fragment
Escherichia coli lac Z gene (lacZ)
Escherichia coli lac Y gene (lacY); fragment
Promoter: Phage λ major leftward promoter (λ PL)
Ribosome
binding site:
Ribosome binding site (RBS) of the phage MS2 polymerase gene
Terminator: -
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli; use strains with a cI function, cIts for PL controlled expression
Parental clone: pPLc236; pPLcZCYA1
Further information: This plasmid contains the coding sequence for a fusion protein between the first 98 amino acids of MS2 polymerase and β-galactosidase.
Expression of a carboxy terminal lacZ and an amino terminal MS2 polymerase (98 amino acids) fused protein is possible via the BamHI site (phase for both fusions: GAT).
The lacY gene is not complete but still functional.
The natural EcoRI site at the end of lacZ was removed by mutation.
Other name of the plasmid is pPLc236LacdelYPol12.
EMBL Accession number: -
Latest sequence update: 01/02/1989
Sequence detail:
Nucleotide sequence at the junction of the MS2 polymerase gene and the lacZ gene:

    98          9   10
5' TGG.GAT.CCC.GTC.GTT 3'
   * BamHI     ^

*: 98th codon of the MS2 polymerase gene.
^: 9th codon of the lacZ gene.
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by E. Saman(1) and Prof. Dr E. Remaut(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061[pRK248cIts]
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Helper plasmid: pRK248cIts
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 28°C
Biosafety level: L1
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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