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LMBP plasmid details

Last data update: 24 November 2020 04:18 CET

Plasmid name: pPLcMIFNG5 (LMBP 1600)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p1600.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Mouse interferon γ cDNA (IFNG); (slightly modified) mature sequence
Promoter: Phage λ major leftward promoter (λ PL)
Ribosome
binding site:
Ribosome binding site (RBS) of the Escherichia coli tryptophan operon attenuator peptide (trp)
Terminator: Phage fd terminator
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Phage f1 origin
Host range: Escherichia coli; use strains with a cI function, cIts for PL controlled expression
Parental clone: pMc58; pPLcMIFNG
Further information: The plasmid was constructed by inserting a BglI-EcoRI fragment from pPLcMIFNG (containing the PL promoter and the mouse IFNG gene) in the unique EcoRI and unique BglI sites of pMc58.
pPLcMFNG5 is an expression plasmid, designed for PL-controlled expression of mature mouse IFNG.
The nucleotide sequence of the mouse IFNG cDNA corresponds with the EMBL Nucleotide Sequence Database accession number K00083.1, except for the slightly modified 5' side.
Other name of the plasmid is pPLcM58MIFNGFdT.
EMBL Accession number: K00083.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 29/05/1989
Sequence detail:
signal sequence ->|-> mature mouse IFNG 
                 Val Ser Gly Cys Tyr Cys His Gly Thr Val Ile Glu Ser Leu Glu Ser Leu       
wild type:  5'...GTT.TCT.GGC|TGT.TAC.TGC.CAC.GGC.ACA.GTC.ATT.GAA.AGC.CTA.GAA.AGT.CTG...3' 
                                                                     MaeI 

                                     Met His Gly Thr Val Ile Glu Ser Leu Glu Ser Leu
pPLcMIFNG5: 5'...TAAAAAGGGTATCGATTCC.ATG.CAC.GGC.ACA.GTC.ATT.GAA.AGC.CTA.GAA.AGT.CTG...3'
                                   -----------------------------------
                                            linker                   MaeI
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr E. Remaut(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061(λ)
Host reference: Mertens et al., Gene 164 (1995), 9-15 [PMID: 7590329]
Related host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 28°C
Biosafety level: L1
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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