Last data update: 24 November 2020 04:18 CET
Plasmid name: pPLcMIFNG (LMBP 1598)
|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner non-core plasmid|
|Cloned DNA:||Mouse interferon γ cDNA (IFNG); (slightly modified) mature sequence|
|Promoter:||Phage λ major leftward promoter (λ PL)|
|Ribosome binding site (RBS) of the Escherichia coli tryptophan operon attenuator peptide (trp)|
|Selection marker:||Ampicillin (amp)|
|Replicon:||Escherichia coli plasmid pMB1 origin|
|Host range:||Escherichia coli; use strains with a cI function, cIts for PL controlled expression|
|Parental clone:||pPLc321; pATMIFNG|
|Further information:||The plasmid was constructed as follows: (i) the MaeI-Sau3AI fragment (mouse mature IFNG fragment + 3UTR) from pATMIFNG was isolated, and ligated with a synthetic sequence (26 bp) at the MaeI site (restoring 7 of the first 10 amino acids of the mature mouse IFNG CDS and adding a NcoI compatible sequence);(ii) this fragment was inserted between the NcoI and BamHI sites of pPLc321.
pPLcMFNG is an expression plasmid, designed for PL-controlled expression of mature mouse IFNG.
The length of the 3' untranslated region of the mouse IFNγ insert is not completely known.
The nucleotide sequence of the mouse IFNG cDNA corresponds with the EMBL Nucleotide Sequence Database accession number K00083.1, except for the slightly modified 5' side.
Other name of the plasmid is pPLcMIFNG15.
|EMBL Accession number:||K00083.1, view at EMBL, GenBank, DDBJ|
|Latest sequence update:||26/05/1989|
signal sequence ->|-> mature mouse IFNG Val Ser Gly Cys Tyr Cys His Gly Thr Val Ile Glu Ser Leu Glu Ser Leu wild type: 5'...GTT.TCT.GGC|TGT.TAC.TGC.CAC.GGC.ACA.GTC.ATT.GAA.AGC.CTA.GAA.AGT.CTG...3' MaeI Met His Gly Thr Val Ile Glu Ser Leu Glu Ser Leu pPLcMIFNG: 5'...TAAAAAGGGTATCGATTCC.ATG.CAC.GGC.ACA.GTC.ATT.GAA.AGC.CTA.GAA.AGT.CTG...3' ClaI ----------------------------------- linker MaeI
|Authenticity test:||The plasmid still needs to be subjected to the authenticity test.|
|History of deposit:||This plasmid was deposited by Prof. Dr E. Remaut(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 MC1061[pcI857]|
|Host reference:||Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
|Cultivation medium:||LB-Lennox + ampicillin (100 μg/ml) + kanamycin (50 μg/ml)|
|Other culture collection numbers:||-|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.