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LMBP plasmid details

Last data update: 24 November 2020 04:18 CET

Plasmid name: pPLcHIFNB31 (LMBP 1650)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p1650.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Human interferon β cDNA (IFNB, IFNB1); fragment of the mature sequence
Promoter: Phage λ major leftward promoter (λ PL)
Ribosome
binding site:
-
Terminator: -
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli; use strains with a cI function, cIts for PL controlled expression
Parental clone: pPLc28; pPLa-HFIF-67-1
Further information: The plasmid was constructed by inserting a fragment of the sequence coding for mature, human IFNB, provided with an EcoRI linker, as an EcoRI-BglII fragment between the EcoRI and BamHI sites of pPLc28. The coding sequence of human IFNB is not complete, it starts at the 2nd codon of the mature sequence.
The nucleotide sequence of the hIFNβ cDNA corresponds with the EMBL Nucleotide Sequence Database accession number V00546.1.
Other names of the plasmid are pBX32-1 and pPLcHFIF31-1.
EMBL Accession number: V00546.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 07/12/1993
Sequence detail:
wild type : signal sequence-->| mature human IFNB  
                                1   2   3   4
               ACA GCT CTT TCC ATG AGC TAC AAC
               Thr Ala Leu Ser Met Ser Tyr Asn

pPLcHIFNB31 :                       2   3   4 
                   GAATTCCCCTCGAGG AGC TAC AAC
                   EcoRI           Ser Tyr Asn
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr E. Remaut(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: Derynck et al., Nature 287 (1980), 193-197 [PMID: 6159534]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061(λ)
Host reference: Mertens et al., Gene 164 (1995), 9-15 [PMID: 7590329]
Related host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 28°C
Biosafety level: L1
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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