LMBP plasmid details

Last data update: 24 November 2020 04:18 CET

Plasmid name: pPLcAT141HIFNB (LMBP 782)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: not available
analysis results


Cloned DNA: Human interferon β cDNA (IFNB, IFNB1); mature sequence
Promoter: Phage λ major leftward promoter (λ PL)
binding site:
Ribosome binding site (RBS); synthetic sequence
Terminator: -
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli; use strains with a cI function, cIts for PL controlled expression
Parental clone: pPLcAT14HIFNB
Further information: pPLcATnHIFNB plasmids are all structural variants of the prototype ribosome binding site and were constructed to test the relevance of this feature for efficient initiation of translation.
pPLcAT141HIFNB is a human IFNB expression plasmid.
This plasmid belongs to the pPLcATnHIFNB series.
This plasmid contains a 52 bp AluI fragment from human IFNB (coordinates 5 to 57) before the SD sequence.
The distance between SD and ATG codon is 8 bases (filled in XbaI site) which abolishes completely the IFNB synthesis. All other pPLcATnHIFNB plasmids have 4 nucleotides between SD and ATG codon.
The nucleotide sequence of the human IFNβ DNA corresponds with the EMBL Nucleotide Sequence Database accession number V00546.1.
EMBL Accession number: V00546.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 17/04/1987
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr E. Remaut(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: Stanssens et al., Gene 36 (1985), 211-223 [PMID: 3000873]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 ΔH1Δtrp
Host reference: Bernard et al., Gene 5 (1979), 59-76 [PMID: 372049]
Related host reference: Remaut et al., Gene 15 (1981), 81-93 [PMID: 6271633]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 28°C
Biosafety level: L1
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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