Last data update: 24 November 2020 04:18 CET
Plasmid name: pPLcAT141HIFNB (LMBP 782)
|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner non-core plasmid|
|Depositor's sequence:||not available|
|Cloned DNA:||Human interferon β cDNA (IFNB, IFNB1); mature sequence|
|Promoter:||Phage λ major leftward promoter (λ PL)|
|Ribosome binding site (RBS); synthetic sequence|
|Selection marker:||Ampicillin (amp)|
|Replicon:||Escherichia coli plasmid pMB1 origin|
|Host range:||Escherichia coli; use strains with a cI function, cIts for PL controlled expression|
|Further information:||pPLcATnHIFNB plasmids are all structural variants of the prototype ribosome binding site and were constructed to test the relevance of this feature for efficient initiation of translation.
pPLcAT141HIFNB is a human IFNB expression plasmid.
This plasmid belongs to the pPLcATnHIFNB series.
This plasmid contains a 52 bp AluI fragment from human IFNB (coordinates 5 to 57) before the SD sequence.
The distance between SD and ATG codon is 8 bases (filled in XbaI site) which abolishes completely the IFNB synthesis. All other pPLcATnHIFNB plasmids have 4 nucleotides between SD and ATG codon.
The nucleotide sequence of the human IFNβ DNA corresponds with the EMBL Nucleotide Sequence Database accession number V00546.1.
|EMBL Accession number:||V00546.1, view at EMBL, GenBank, DDBJ|
|Latest sequence update:||17/04/1987|
|Authenticity test:||The plasmid still needs to be subjected to the authenticity test.|
|History of deposit:||This plasmid was deposited by Prof. Dr E. Remaut(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
|Plasmid reference:||Stanssens et al., Gene 36 (1985), 211-223 [PMID: 3000873]
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 ΔH1Δtrp|
|Host reference:||Bernard et al., Gene 5 (1979), 59-76 [PMID: 372049]
|Related host reference:||Remaut et al., Gene 15 (1981), 81-93 [PMID: 6271633]
|Cultivation medium:||LB-Lennox + ampicillin (100 μg/ml)|
|Other culture collection numbers:||-|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.