Last data update: 24 November 2020 04:18 CET
Plasmid name: pPLc436HIL2 (LMBP 962)
|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner non-core plasmid|
|Cloned DNA:||Human interleukin 2 cDNA (IL2); mature sequence|
|Promoter:||Phage λ major leftward promoter (λ PL)|
|Ribosome binding site (RBS) of the phage Mu ner protein gene|
|Selection marker:||Ampicillin (amp)|
|Replicon:||Escherichia coli plasmid pMB1 origin|
|Host range:||Escherichia coli; use strains with a cI function|
|Parental clone:||pAT153HIL2B; pGB299|
|Further information:||pPLc436HIL2 is an expression plasmid, designed for expression of mature human IL2.
There is a correct fusion between the mature human IL2 sequence and the ATG codon and the phage Mu ner protein gene Shine-Dalgarno region supplied by pGB299.
The StuI site after the human IL2 gene (base position 496) can be used as a unique site; the second StuI site (base position 4818) cuts only in dcm-negative strains.
The nucleotide sequence of the human IL2 corresponds with the EMBL Nucleotide Sequence Database accession number X01586.1, except for the first codon of the mature sequence (GCA) that is GCG.
The nucleotide sequence of the phage Mu ner protein 5' UTR corresponds with the EMBL Nucleotide Sequence Database accession number X01586.1.
Other names of the plasmid are pPLcMuHIL201 and pPLc436MUHIL201.
|EMBL Accession number:||X01586.1, view at EMBL, GenBank, DDBJ
M10192.1, view at EMBL, GenBank, DDBJ
|Latest sequence update:||12/06/1990|
|Authenticity test:||The plasmid still needs to be subjected to the authenticity test.|
|History of deposit:||This plasmid was deposited by Prof. Dr E. Remaut(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
|Plasmid reference:||Devos et al., Nucleic Acids Res. 11 (1983), 4307-4323 [PMID: 6306584]
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 ΔH1Δtrp|
|Host reference:||Bernard et al., Gene 5 (1979), 59-76 [PMID: 372049]
|Related host reference:||Remaut et al., Gene 15 (1981), 81-93 [PMID: 6271633]
|Cultivation medium:||LB-Lennox + ampicillin (100 μg/ml)|
|Other culture collection numbers:||-|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.