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LMBP plasmid details

Last data update: 24 November 2020 04:18 CET

Plasmid name: pPLc436HIL2 (LMBP 962)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p962.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Human interleukin 2 cDNA (IL2); mature sequence
Promoter: Phage λ major leftward promoter (λ PL)
Ribosome
binding site:
Ribosome binding site (RBS) of the phage Mu ner protein gene
Terminator: -
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli; use strains with a cI function
Parental clone: pAT153HIL2B; pGB299
Further information: pPLc436HIL2 is an expression plasmid, designed for expression of mature human IL2.
There is a correct fusion between the mature human IL2 sequence and the ATG codon and the phage Mu ner protein gene Shine-Dalgarno region supplied by pGB299.
The StuI site after the human IL2 gene (base position 496) can be used as a unique site; the second StuI site (base position 4818) cuts only in dcm-negative strains.
The nucleotide sequence of the human IL2 corresponds with the EMBL Nucleotide Sequence Database accession number X01586.1, except for the first codon of the mature sequence (GCA) that is GCG.
The nucleotide sequence of the phage Mu ner protein 5' UTR corresponds with the EMBL Nucleotide Sequence Database accession number X01586.1.
Other names of the plasmid are pPLcMuHIL201 and pPLc436MUHIL201.
EMBL Accession number: X01586.1, view at EMBL, GenBank, DDBJ
M10192.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 12/06/1990
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr E. Remaut(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: Devos et al., Nucleic Acids Res. 11 (1983), 4307-4323 [PMID: 6306584]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 ΔH1Δtrp
Host reference: Bernard et al., Gene 5 (1979), 59-76 [PMID: 372049]
Related host reference: Remaut et al., Gene 15 (1981), 81-93 [PMID: 6271633]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 28°C
Biosafety level: L1
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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