Last data update: 24 November 2020 04:18 CET
Plasmid name: pPLc428T1HIL2 (LMBP 966)
|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner non-core plasmid|
|Cloned DNA:||Human interleukin 2 cDNA (IL2); mature sequence|
|Promoter:||Phage λ major leftward promoter (λ PL)|
|Ribosome binding site (RBS) of the phage Mu ner protein gene|
|Terminator:||Phage T4 gene 32 terminator (T4g32)|
|Selection marker:||Ampicillin (amp)|
|Replicon:||Escherichia coli plasmid pMB1 origin|
|Host range:||Escherichia coli; use strains with a cI function, cIts for PL controlled expression|
|Parental clone:||pPLc28; pPLc436HIL2|
|Further information:||The plasmid is meant for expression of hIL2.
The 3' untranslated region of the hIL2 gene is followed by GC tails, the length of which is not known.
The nucleotide sequence of the human IL2 corresponds with the EMBL Nucleotide Sequence Database accession number X01586.1, except for the first codon of the mature sequence (GCA) that is replaced by an ATG.
Other name of the plasmid is pPLcMuHIL202-T4
|EMBL Accession number:||X01586.1, view at EMBL, GenBank, DDBJ|
|Latest sequence update:||09/07/1990|
|Authenticity test:||The plasmid still needs to be subjected to the authenticity test.|
|History of deposit:||This plasmid was deposited by Prof. Dr E. Remaut(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 ΔH1Δtrp|
|Host reference:||Bernard et al., Gene 5 (1979), 59-76 [PMID: 372049]
|Related host reference:||Remaut et al., Gene 15 (1981), 81-93 [PMID: 6271633]
|Cultivation medium:||LB-Lennox + ampicillin (100 μg/ml)|
|Other culture collection numbers:||-|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.