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LMBP plasmid details

Last data update: 24 November 2020 04:18 CET

Plasmid name: pPLc328T1HIL2 (LMBP 968)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p968.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Human interleukin 2 cDNA (IL2); mature sequence
Promoter: Phage λ major leftward promoter (λ PL)
Ribosome
binding site:
Ribosome binding site (RBS) of the Escherichia coli tryptophan operon attenuator peptide (trp)
Terminator: Phage T4 gene 32 terminator (T4g32)
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli; use strains with a cI function, cIts for PL controlled expression
Parental clone: pTrp336HIL2
Further information: pPLc436HIL2 is a human IL2 expression plasmid.
The mature human IL2 is preceded by the RBS of the E. coli tryptophan operon attenuator and followed by the phage T4 gene 32 terminator.
The StuI site after the human IL2 gene (base position 496) can be used as a unique site; the second StuI site (base position 4818) cuts only in dcm-negative strains.
The nucleotide sequence of the human IL2 corresponds with the EMBL Nucleotide Sequence Database accession number X01586.1, except for the first codon of the mature sequence (GCA) that is replaced by an ATG.
Other name of the plasmid is pPLc28TRPHIL202T4.
EMBL Accession number: X01586.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 12/01/1987
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr E. Remaut(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: Devos et al., Nucleic Acids Res. 11 (1983), 4307-4323 [PMID: 6306584]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 DH5α
Host reference: Focus 8 (1986), 9
Related host reference: Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051]
Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187]
Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791]
Rodriguez-Quinones et al., Focus 15 (1993), 110-112
Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 28°C
Biosafety level: L1
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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