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LMBP plasmid details

Last data update: 24 November 2020 04:18 CET

Plasmid name: pPLc321T7C10T (LMBP 1539)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p1539.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Phage T7 gene 10 (T7g10) encoding A protein (without frameshift)
Phage T7 gene 10 (T7g10) encoding B protein (with frameshift)
Promoter: Phage λ major leftward promoter (λ PL)
Phage T7 gene 10 promoter (T7g10)
Ribosome
binding site:
Ribosome binding site (RBS) of the Escherichia coli tryptophan operon attenuator peptide (trp)
Ribosome binding site (RBS) of the phage T7 gene 10 (T7g10)
Terminator: Phage T7 gene 10 terminator (T7g10)
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli; use strains with a cI function, cIts for PL controlled expression
Parental clone: pPLc321
Further information: This plasmid contains phage T7 gene number 10 that codes for the T7 major head protein; the protein can be expressed either from the PL promoter or from the T7 promoter (the latter dependent on the T7 RNA polymerase). The coding region has a frameshift of 2 nucleotides at the end.
The protein without the frameshift is called the A protein; the protein with the frameshift is called the B protein.
EMBL Accession number: -
Latest sequence update: 01/10/1990
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr N. Mertens(1) and Prof. Dr E. Remaut(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 ΔH1Δtrp
Host reference: Bernard et al., Gene 5 (1979), 59-76 [PMID: 372049]
Related host reference: Remaut et al., Gene 15 (1981), 81-93 [PMID: 6271633]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 28°C
Biosafety level: L1
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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