Last data update: 24 November 2020 04:18 CET
Plasmid name: pPLc321T7C10T (LMBP 1539)
|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner non-core plasmid|
|Cloned DNA:||Phage T7 gene 10 (T7g10) encoding A protein (without frameshift)
Phage T7 gene 10 (T7g10) encoding B protein (with frameshift)
|Promoter:||Phage λ major leftward promoter (λ PL)
Phage T7 gene 10 promoter (T7g10)
|Ribosome binding site (RBS) of the Escherichia coli tryptophan operon attenuator peptide (trp)
Ribosome binding site (RBS) of the phage T7 gene 10 (T7g10)
|Terminator:||Phage T7 gene 10 terminator (T7g10)|
|Selection marker:||Ampicillin (amp)|
|Replicon:||Escherichia coli plasmid pMB1 origin|
|Host range:||Escherichia coli; use strains with a cI function, cIts for PL controlled expression|
|Further information:||This plasmid contains phage T7 gene number 10 that codes for the T7 major head protein; the protein can be expressed either from the PL promoter or from the T7 promoter (the latter dependent on the T7 RNA polymerase). The coding region has a frameshift of 2 nucleotides at the end.
The protein without the frameshift is called the A protein; the protein with the frameshift is called the B protein.
|EMBL Accession number:||-|
|Latest sequence update:||01/10/1990|
|Authenticity test:||The plasmid still needs to be subjected to the authenticity test.|
|History of deposit:||This plasmid was deposited by Dr N. Mertens(1) and Prof. Dr E. Remaut(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 ΔH1Δtrp|
|Host reference:||Bernard et al., Gene 5 (1979), 59-76 [PMID: 372049]
|Related host reference:||Remaut et al., Gene 15 (1981), 81-93 [PMID: 6271633]
|Cultivation medium:||LB-Lennox + ampicillin (100 μg/ml)|
|Other culture collection numbers:||-|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.