Last data update: 24 November 2020 04:18 CET
Plasmid name: pPLc321MIL2 (LMBP 1448)
|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner non-core plasmid|
|Cloned DNA:||Mouse interleukin 2 cDNA (IL2); mature sequence|
|Promoter:||Phage λ major leftward promoter (λ PL)|
|Ribosome binding site (RBS) of the Escherichia coli tryptophan operon attenuator peptide (trp)|
|Selection marker:||Ampicillin (amp)|
|Replicon:||Escherichia coli plasmid pMB1 origin|
|Host range:||Escherichia coli; use strains with a cI function, cIts for PL controlled expression|
|Parental clone:||pPLc321; pTrp321MIL2|
|Further information:||pPLc321MIL2 is an expression plasmid, designed for expression of mature mouse IL2.
The complete coding sequence of the tetracycline resistance gene is present, but its expression was not experimentally verified.
The nucleotide sequence of the mouse IL2 DNA corresponds with the EMBL Nucleotide Sequence Database accession number X01772.1.
|EMBL Accession number:||X01772.1, view at EMBL, GenBank, DDBJ|
|Latest sequence update:||15/03/1990|
|Authenticity test:||The plasmid still needs to be subjected to the authenticity test.|
|History of deposit:||This plasmid was deposited by Prof. Dr E. Remaut(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
|Plasmid reference:||PhD thesis Jan Demolder (1986)
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 ΔH1trp|
|Cultivation medium:||LB-Lennox + ampicillin (100 μg/ml)|
|Other culture collection numbers:||-|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.