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LMBP plasmid details

Last data update: 24 November 2020 04:18 CET

Plasmid name: pPLc321MIL2 (LMBP 1448)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p1448.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Mouse interleukin 2 cDNA (IL2); mature sequence
Promoter: Phage λ major leftward promoter (λ PL)
Ribosome
binding site:
Ribosome binding site (RBS) of the Escherichia coli tryptophan operon attenuator peptide (trp)
Terminator: -
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli; use strains with a cI function, cIts for PL controlled expression
Parental clone: pPLc321; pTrp321MIL2
Further information: pPLc321MIL2 is an expression plasmid, designed for expression of mature mouse IL2.
The complete coding sequence of the tetracycline resistance gene is present, but its expression was not experimentally verified.
The nucleotide sequence of the mouse IL2 DNA corresponds with the EMBL Nucleotide Sequence Database accession number X01772.1.
EMBL Accession number: X01772.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 15/03/1990
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr E. Remaut(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: PhD thesis Jan Demolder (1986)
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 ΔH1trp
Host reference: -
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 28°C
Biosafety level: L1
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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