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LMBP plasmid details

Last data update: 24 November 2020 04:18 CET

Plasmid name: pPLc321K1 (LMBP 1480)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p1480.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Escherichia coli plasmid pMB9 kil gene
Promoter: Phage λ major leftward promoter (λ PL)
Ribosome
binding site:
Ribosome binding site (RBS) of the Escherichia coli tryptophan operon attenuator peptide (trp)
Terminator: -
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli; use strains with a cI function, cIts for PL controlled expression
Parental clone: pPLc321; pMB9
Further information: The kil gene expression causes release of periplasmic proteins and cell death.
The nucleotide sequence of the kil gene corresponds with the EMBL Nucleotide Sequence Database accession number M13468.1.
EMBL Accession number: M13468.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 27/11/1991
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr L. Steidler(1) and Prof. Dr E. Remaut(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: Steidler et al., Biotechnol. Bioeng. 44 (1994), 1074-1082 [PMID: 18623024]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 ΔH1Δtrp
Host reference: Bernard et al., Gene 5 (1979), 59-76 [PMID: 372049]
Related host reference: Remaut et al., Gene 15 (1981), 81-93 [PMID: 6271633]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 28°C
Biosafety level: L1
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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