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LMBP plasmid details

Last data update: 24 November 2020 04:18 CET

Plasmid name: pPLc321 (LMBP 1436)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: pplc321.gb (View with Genome Compiler)
pplc321.pdf
Sequence
analysis results
Genecorner:

-

Cloned DNA: -
Promoter: Phage λ major leftward promoter (λ PL)
Ribosome
binding site:
Ribosome binding site (RBS) of the Escherichia coli tryptophan operon attenuator peptide (trp)
Terminator: -
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli; use strains with a cI function, cIts for PL controlled expression
Parental clone: pTrp321; pPLc28
Further information: Designed for λ PL promoter-regulated expression of nonfused proteins after NcoI digestion and filling in the 5' sticky ends.
Can also be used for the expression of fused proteins after HindIII or BalI digestion.
The BalI site at nucleotide position 41 can be used as a unique site: the other BalI site (nucleotide position 1461) cuts only in dcm-negative strains.
It is not experimentally verified whether this plasmid still provides resistance to tetracycline (the nucleotide sequence from the HindIII site clockwise to the ampicillin resistance gene originates from pBR322).
EMBL Accession number: -
Latest sequence update: 25/01/1989
Sequence detail:
Nucleotide sequence around the trp Shine-Dalgarno position:


5' AAAAAGGGTATCGATTCC ATG.GCC.AAG.CTT. 3'
     -----  ClaI   NcoI       HindIII
      SD               BalI


SD: Shine-Dalgarno position.
Punctuation indicates reading frame.
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: BalI, BamHI and HindII.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr E. Remaut(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061(λ)
Host reference: Mertens et al., Gene 164 (1995), 9-15 [PMID: 7590329]
Related host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 28°C
Biosafety level: L1
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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