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LMBP plasmid details

Last data update: 24 November 2020 04:18 CET

Plasmid name: pPLc299HIL4 (LMBP 1923)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p1923.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Human interleukin 4 cDNA (IL4)
Promoter: Phage λ major leftward promoter (λ PL)
Ribosome
binding site:
Ribosome binding site (RBS) of the phage Mu ner protein gene
Terminator: -
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli; use strains with a cI function, cIts for PL controlled expression
Parental clone: pGB299; pSP65HIL4
Further information: The plasmid was constructed as follows : the EcoRV-BamHI human IL4 fragment was isolated from pSP65HIL4 and a linker ('CATGCACAAGTGCGAT') was ligated to the EcoRV site : this linker reconstitutes the mature human IL4 gene and introduces aninitiating AUG which is also part of a NcoI site. The NcoI-BamHI fragment was inserted between the unique NcoI and U-BamHI sites of pGB299.
The first 72 nucleotides of the human IL4 region corresponding to the signal sequence are missing.
The publication of Yokota et. al. (1986) was used to decide where to place the AUG start codon of human IL4.
EMBL Accession number: -
Latest sequence update: 22/02/1989
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr E. Remaut(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Related plasmid reference: Yokota et al., Proc. Natl. Acad. Sci. U.S.A. 83 (1986), 5894-5898 [PMID: 3016727]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061[pcI857]
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Helper plasmid: pcI857
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml) + kanamycin (50 μg/ml)
Cultivation temperature: 28°C
Biosafety level: L1
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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