LMBP plasmid details

Last data update: 24 November 2020 04:18 CET

Plasmid name: pPLc28LIG8 (LMBP 574)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: (View with Genome Compiler)
analysis results


Cloned DNA: Phage T4 ligase gene
Promoter: Phage λ major leftward promoter (λ PL)
binding site:
Ribosome binding site (RBS) of the phage T4 ligase gene
Terminator: -
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli; use strains with a cI function, cIts for PL controlled expression
Parental clone: pPLc28; λNM540T4lig
Further information: The plasmid contains the T4 ligase enzyme coding region, cloned as a HindIII fragment from phage λNM540T4lig (Wilson et al. (1979)) in the HindIII site of pPLc28, in the sense orientation relative to the λ PL promoter.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT726946.1.
The nucleotide sequence of the phage T4 ligase gene corresponds with the EMBL Nucleotide Sequence Database accession number X00039.1.
Name mentioned in Remaut et al. (1983) is pPLc28lig8.
Other name of the plasmid is pST28lig8.
EMBL Accession number: X00039.1, view at EMBL, GenBank, DDBJ
LT726946.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 25/03/1992
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: BglII, EcoRI, HindIII and XmnI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr E. Remaut(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: Remaut et al., Gene 22 (1983), 103-113 [PMID: 6305768]
Related plasmid reference: Wilson et al., J. Mol. Biol. 132 (1979), 471-491 [PMID: 160464]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 C600[pcI857]
Host reference: -
Related host reference: Appleyard, Genetics 39 (1954), 440-452 [PMID: 17247495]
Helper plasmid: pcI857
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml) + kanamycin (50 μg/ml)
Cultivation temperature: 28°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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