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LMBP plasmid details

Last data update: 24 November 2020 04:18 CET

Plasmid name: pPLc28LIG15 (LMBP 501)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p501.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Phage T4 ligase gene
Promoter: Phage λ major leftward promoter (λ PL)
Ribosome
binding site:
Ribosome binding site (RBS) of the phage T4 ligase gene
Terminator: -
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli; use strains with a cI function, cIts for PL controlled expression
Parental clone: pPLc28; λNM540T4lig
Further information: This plasmid contains the T4 ligase enzyme coding region, cloned as a HindIII fragment in the HindIII site of pPLc28, in the antisense orientation relative to the λ PL promoter.
Name mentioned in Remaut et al. (1983) is pPLc28lig15.
Other name of the plasmid is pST28lig15.
EMBL Accession number: -
Latest sequence update: 25/03/1992
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr E. Remaut(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: Remaut et al., Gene 22 (1983), 103-113 [PMID: 6305768]
Related plasmid reference: Wilson et al., J. Mol. Biol. 132 (1979), 471-491 [PMID: 160464]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 r-m+ (λ)
Host reference: -
Cultivation medium: LB-Lennox
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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