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LMBP plasmid details

Last data update: 24 November 2020 04:18 CET

Plasmid name: pPLc2833 (LMBP 483)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: pplc2833.gb (View with Genome Compiler)
pplc2833.pdf
Sequence
analysis results
Genecorner:

-

Cloned DNA: -
Promoter: Phage λ major leftward promoter (λ PL)
Ribosome
binding site:
-
Terminator: -
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli; use strains with a cI function, cIts for PL controlled expression
Parental clone: pPLc28
Further information: The plasmid is designed for λ PL promoter-regulated expression of fragments containing a ribosome binding site functional in E. coli.
This plasmid carries two BamHI, two SalI and three XbaI expression sites in the polylinker downstream from the λ PL promoter.
The PstI expression site is not unique: there is another one in the ampicillin resistance gene.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727045.1.
Other name of the plasmid is pST28-33.
EMBL Accession number: LT727045.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 03/04/1989
Sequence detail:
Nucleotide sequence of the polylinker downstream from the PL promoter:


5' GAATTCCGGATCCGGCTCTAGAGGGTCGACCCTCTAGAGGGTCGACCCTCTAGAGGCTGCAGCCGA
   EcoRI  BamHI    XbaI    SalI    XbaI    SalI    XbaI    PstI
    BspMII

CCCCGGATCCGGCCAAGCTT 3'
       BamHI     HindIII
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: HaeII, PstI and PvuI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr E. Remaut(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: Remaut et al., Gene 22 (1983), 103-113 [PMID: 6305768]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 r-m+ (λ)
Host reference: -
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 28°C
Biosafety level: L1
Other culture collection numbers: NCCB 3065
PC-V 3065

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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