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LMBP plasmid details

Last data update: 24 November 2020 04:18 CET

Plasmid name: pPLc2820BS (LMBP 836)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p836.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Ampicillin resistance gene (amp); signal sequence
Promoter: Escherichia coli β-lactamase promoter (amp)
Phage λ major leftward promoter (λ PL)
Ribosome
binding site:
Ribosome binding site (RBS) of the Escherichia coli ampicillin resistance gene (amp)
Terminator: -
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli; use strains with a cI function, cIts for PL controlled expression
Parental clone: pPLc2820B
Further information: The SalI site can be used for expression of proteins that are fused close to the amp signal protein.
XbaI and HindIII cannot be used for expression of fused proteins: the XbaI site contains a termination codon in phase.
HindII site is not an unique site.
The amp promoter is not controllable.
Other name of the plasmid is pPLc28-20ß279S.
EMBL Accession number: -
Latest sequence update: 08/06/1990
Sequence detail:
Nucleotide sequence of the ampicillin gene with the polylinker:

5' ATG.AGT.ATT.CAA.CAT.TTC.CGT.GTC.GCC.CTT.ATT.CCC.TTT.TTT.GCG.
   *  
    
                                               @
   GCA.TTT.TGC.CTT.CCT.GTT.TTT.GCG.GTC.GAC.CTC.TAG AGC.CAA.GCT.T 3'
                                   ^        XbaI        HindIII
                                   SalI
                                   HindII 

*: Start of the amp coding sequence.
^: Start of the mature sequence of the amp gene.
@: Termination codon.
Punctuation indicates reading frame.
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr E. Remaut(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: Leemans et al., Gene 85 (1989), 99-108 [PMID: 2695405]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 K514(λ)
Host reference: Zabeau et al., EMBO J. 1 (1982), 1217-1224 [PMID: 6327257]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 28°C
Biosafety level: L1
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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