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LMBP plasmid details

Last data update: 24 November 2020 04:18 CET

Plasmid name: pPLc2820BHIL2dBH (LMBP 972)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p972.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Human interleukin 2 cDNA (IL2); mature sequence
Ampicillin resistance gene (amp); signal sequence
Promoter: Escherichia coli β-lactamase promoter (amp)
Phage λ major leftward promoter (λ PL)
Ribosome
binding site:
Ribosome binding site (RBS) of the Escherichia coli ampicillin resistance gene (amp)
Terminator: -
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli; use strains with a cI function, cIts for PL controlled expression
Parental clone: pPLc2820BIL
Further information: pPLc2820BHIL2dBH is an expression plasmid, designed for expression and secretion of human IL2.
The mature human IL2 has a correct fusion with the signal sequence of the ampicillin resistance gene.
The plasmid is 342 bp smaller than pPLc2820BHIL2. The unique BamHI and HindIII sites of pPLc2820BHIL2 were cut, filled in and religated.
The nucleotide sequence of the human IL2 corresponds with the EMBL Nucleotide Sequence Database accession number X01586.1, except for the first codon of the mature sequence (GCA) that is GCG.
Other name of the plasmid is pPLc28-20betaNIL2delBH.
EMBL Accession number: X01586.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 03/02/1987
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr E. Remaut(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 K514(λ)
Host reference: Zabeau et al., EMBO J. 1 (1982), 1217-1224 [PMID: 6327257]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 28°C
Biosafety level: L1
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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