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LMBP plasmid details

Last data update: 24 November 2020 04:18 CET

Plasmid name: pPLc2820B (LMBP 835)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p835.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Ampicillin resistance gene (amp); signal sequence
Promoter: Escherichia coli β-lactamase promoter (amp)
Phage λ major leftward promoter (λ PL)
Ribosome
binding site:
Ribosome binding site (RBS) of the Escherichia coli ampicillin resistance gene (amp)
Terminator: -
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli; use strains with a cI function, cIts for PL controlled expression
Parental clone: pPLc2820; pKT279
Further information: PstI can be used for expression of proteins that are fused close to the amp signal protein.
XbaI, SalI and HindIII can not be used for the expression of fused proteins: the first XbaI site contains a termination codon in phase.
The amp promoter is not controllable.
Other name of the plasmid is pPLc28-20ß279.
EMBL Accession number: -
Latest sequence update: 08/02/1989
Sequence detail:
Nucleotide sequence of the amp gene with the polylinker:

5' ATG.AGT.ATT.CAA.CAT.TTC.CGT.GTC.GCC.CTT.ATT.CCC.TTT.TTT.GCG.
   *  


                                                       @
   GCA.TTT.TGC.CTT.CCT.GTT.TTT.GCT.CAC.CGC.TGC.AGC.CTC.TAG.AGG.
                                   ^     PstI       XbaI

   GTC.GAC.CTC.TAG.AGC.CAA.GCT.T 3'
   SalI     XbaI        HindIII
   HindII

*: Start of the amp coding sequence.
^: Start of the mature sequence of the amp gene.
@: Termination codon.
Punctuation indicates reading frame.
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr E. Remaut(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: Leemans et al., Gene 85 (1989), 99-108 [PMID: 2695405]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 K514(λ)
Host reference: Zabeau et al., EMBO J. 1 (1982), 1217-1224 [PMID: 6327257]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 28°C
Biosafety level: L1
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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