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LMBP plasmid details

Last data update: 24 November 2020 04:18 CET

Plasmid name: pPLc2820 (LMBP 1442)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: pplc2820.gb (View with Genome Compiler)
pplc2820.pdf
Sequence
analysis results
Genecorner:

-

Cloned DNA: -
Promoter: Phage λ major leftward promoter (λ PL)
Ribosome
binding site:
-
Terminator: -
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli; use strains with a cI function, cIts for PL controlled expression
Parental clone: pPLc2819; pUC8
Further information: The plasmid is designed for expression of fragments containing a ribosome binding site functional in E. coli.
pPLc2820 differs from pPLc2819 in the orientation of the polylinker downstream from the λ-PL promoter and having a unique PstI expression site.
pPLc2820 has no PstI site in the ampicillin resistance gene.
The HindIII and XbaI expression sites are not unique.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727574.1.
EMBL Accession number: LT727574.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 01/02/1989
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: BamHI, HindII, MspA1I and RsaI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by R. Leemans(1) and Prof. Dr E. Remaut(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061(λ)
Host reference: Mertens et al., Gene 164 (1995), 9-15 [PMID: 7590329]
Related host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 28°C
Biosafety level: L1
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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