Last data update: 24 November 2020 04:18 CET
Plasmid name: pPLc2819FdT1 (LMBP 741)
|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner non-core plasmid|
|Promoter:||Phage λ major leftward promoter (λ PL)|
|Terminator:||Phage fd terminator|
|Selection marker:||Ampicillin (amp)|
|Replicon:||Escherichia coli plasmid pMB1 origin|
|Host range:||Escherichia coli; use strains with a cI function, cIts for PL controlled expression|
|Further information:||The plasmid contains fd terminator (fdT) and the lac operator fragment (lacO) flanked by several unique restriction sites, and the in a functional orientation relative to the λ-PL promoter.
The orientation of the 'fdT-lacO' fragment was experimentally verified.
The lac operator fragment is suitable as a screeningsmarker on lacZ indicator plates in lac positive strains.
The HindIII, PstI and XbaI expression sites are not unique.
|EMBL Accession number:||-|
|Latest sequence update:||03/04/1989|
Nucleotide sequence downstream from the PL promoter: 5' GAATTCCGGATCCGGCCAAGCTTGGCTCTAGAGGCTGCAGCCTCTAGAGGGTCGAAATT 3' EcoRI BamHI HindIII XbaI PstI XbaI BspMII
|Authenticity test:||The plasmid still needs to be subjected to the authenticity test.|
|History of deposit:||This plasmid was deposited by Prof. Dr E. Remaut(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli W6 (λrex)|
|Cultivation medium:||LB-Lennox + ampicillin (100 μg/ml)|
|Other culture collection numbers:||-|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.