GREAT AT SMALL THINGS

0

LMBP plasmid details

Last data update: 24 November 2020 04:18 CET

Plasmid name: pPLc245B (LMBP 973)

Add to cart

New search

Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: pplc245b.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Ampicillin resistance gene (amp); signal sequence
Promoter: Phage λ major leftward promoter (λ PL)
Ribosome
binding site:
Ribosome binding site (RBS) of the phage MS2 polymerase gene
Terminator: -
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli; use strains with a cI function, cIts for PL controlled expression
Parental clone: pPLc245; pPLc2820BAH3
Further information: ApaI and HgiJII can be used for the expresssion of proteins that are correctly fused to the amp signal protein via removal of the 3' sticky ends.
AvaI, NarI, and SmaI can be used for the expression of proteins that are correctly fused to the amp mature protein.
E-AvaI and E-SmaI are not unique.
Other name of the plasmid is pPLc2820betaProm-.
EMBL Accession number: -
Latest sequence update: 08/06/1990
Sequence detail:
Nucleotide sequence of the ampicillin gene with the polylinker:

5' ATG.AGT.ATT.CAA.CAT.TTC.CGT.GTC.GCC.CTT.ATT.CCC.TTT.TTT.GCG.
   *

   GCA.TTT.TGC.CTT.CCT.GTT.TTT.GCG.GGC.CCG.GGC.GCC.CGG.GCC.TAG. 
                                   ^            SmaI       @
                                 ApaI      NarI AvaI
                                 HgiJII
                                     SmaI
                                     AvaI

   AGC.CAA.GCT.TGG.CTC.TAG.AGC.CAA.GCT.T 3'
        HindIII     XbaI        HindIII

*: Start codon of the amp gene.
^: Start of the mature sequence of the amp gene.
@: Termination codon.
Punctuation indicates reading frame.
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr E. Remaut(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: Leemans et al., Gene 85 (1989), 99-108 [PMID: 2695405]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 K514(λ)
Host reference: Zabeau et al., EMBO J. 1 (1982), 1217-1224 [PMID: 6327257]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 28°C
Biosafety level: L1
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

New search