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LMBP plasmid details

Last data update: 24 November 2020 04:18 CET

Plasmid name: pPLc245 (LMBP 682)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: pplc245.gb (View with Genome Compiler)
pplc245.pdf
Sequence
analysis results
Genecorner:

-

Cloned DNA: -
Promoter: Phage λ major leftward promoter (λ PL)
Ribosome
binding site:
Ribosome binding site (RBS) of the phage MS2 polymerase gene
Terminator: -
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli; use strains with a cI function, cIts for PL controlled expression
Parental clone: pPLc24; pPLc2819; pPLc28
Further information: Designed for the expression of nonfused proteins after SalI digestion and e.g. S1 nuclease treatment of the 5' sticky ends, making the ATG start codon on the plasmid accessible for blunt-end ligation.
XbaI, PstI and HindIII cannot be used for expression: the first XbaI site contains a termination codon in phase with ATG.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT726843.1.
EMBL Accession number: LT726843.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 02/10/1992
Sequence detail:
See page 2.









Nucleotide sequence at the Shine-Dalgarno (SD) of the MS2 polymerase ribosome binding site:


5' CATGAGGATTACCC ATG.TCG.ACC.CTC.TAG.AGG.CTG.CAG.CCT.CTA.GAG.CCA.AGC.TTG 3'
       ------                      *
        SD          SalI       XbaI       PstI      XbaI        HindIII


*: Termination codon.
Punctuation indicates reading frame.
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: HindII, PvuI, RsaI and TaqI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr E. Remaut(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: Remaut et al., Nucleic Acids Res. 11 (1983), 4677-4688 [PMID: 6308555]
Related plasmid reference: Nishihara et al., J. Gen. Virol. 83 (2002), 2601-2606 [PMID: 12237444] [DOI: 10.1099/0022-1317-83-10-2601]
Nishihara et al., Genes Cells 9 (2004), 877-889 [PMID: 15461660] [DOI: 10.1111/j.1365-2443.2004.00778.x]
Nishihara et al., Microbiol. Immunol. 50 (2006), 61-66 [PMID: 16428875]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 ΔH1Δtrp
Host reference: Bernard et al., Gene 5 (1979), 59-76 [PMID: 372049]
Related host reference: Remaut et al., Gene 15 (1981), 81-93 [PMID: 6271633]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 28°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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