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LMBP plasmid details

Last data update: 24 November 2020 04:18 CET

Plasmid name: pPLc24 (LMBP 399)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: pplc24.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Phage MS2 polymerase gene; first 98 codons
Promoter: Phage λ major leftward promoter (λ PL)
Ribosome
binding site:
Ribosome binding site (RBS) of the phage MS2 polymerase gene
Terminator: -
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli; use strains with a cI function, cIts for PL controlled expression
Parental clone: pPLc28B; pMS27
Further information: Designed for expression of fused proteins with MS2 polymerase.
Insertion sites: BamHI, HindIII.
Other name of the plasmid is pSTL24.
EMBL Accession number: -
Latest sequence update: 31/01/1989
Sequence detail:
Nucleotide sequence around the insertion sites:

        *
5' TCA.TGG.GAT.CCG.GCC.AAG.CTT.GGC.TGC.CTC.GCG....CTC.TGA 3'
         BamHI         HindIII                         ^

* : 98th codon of MS2 polymerase.
^ : Termination codon in phase with the ATG start codon of the MS2 polymerase gene.
Punctuation indicates reading frame.
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr E. Remaut(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: Remaut et al., Gene 15 (1981), 81-93 [PMID: 6271633]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli W6 (λrex)
Host reference: -
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 28°C
Biosafety level: L1
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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