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LMBP plasmid details

Last data update: 24 November 2020 04:18 CET

Plasmid name: pPLc23trpA1 (LMBP 442)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: p442.gb (View with Genome Compiler)
p442.pdf
Sequence
analysis results
Genecorner:

-

Cloned DNA: Salmonella enterica serovar Typhimurium tryptophan synthetase A gene (trpA)
Promoter: Phage λ major leftward promoter (λ PL)
Ribosome
binding site:
-
Terminator: -
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli; use strains with a cI function, cIts for PL controlled expression
Parental clone: pPLc236; pES9
Further information: This plasmid was constructed by inserting the EcoRI-SalI fragment from pES9 between the EcoRI and SalI sites of pPLc236. This fragment contains the Salmonella typhimurium tryptophan synthetase A gene (trpA) as well as the larger part of the S. typhimurium trpB gene, missing its translation initiation region.
The nucleotide sequence of the S. typhimurium fragment corresponds with the Genbank accession number NC_003197.1.
Other name of the plasmid is pST23TrpA11.
EMBL Accession number: NC_003197.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 07/01/2003
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: NruI, PvuI and SalI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr E. Remaut (1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: Remaut et al., Gene 15 (1981), 81-93 [PMID: 6271633]
Related plasmid reference: Nichols et al., J. Mol. Biol. 142 (1980), 503-517 [PMID: 7007652]
McClelland et al., Nature 413 (2001), 852-856 [PMID: 11677609]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 M5219
Host reference: Remaut et al., Gene 15 (1981), 81-93 [PMID: 6271633]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 28°C
Biosafety level: L1
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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