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LMBP plasmid details

Last data update: 24 November 2020 04:18 CET

Plasmid name: pPLc236 (LMBP 379)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: pplc236.gb (View with Genome Compiler)
pplc236.pdf
Sequence
analysis results
Genecorner:

-

Cloned DNA: -
Promoter: Phage λ major leftward promoter (λ PL)
Ribosome
binding site:
-
Terminator: -
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli; use strains with a cI function, cIts for PL controlled expression
Parental clone: pPLc23
Further information: Designed for λ PL promoter-regulated expression of fragments containing a ribosome binding site functional in Escherichia coli.
Expression sites: BalI, BamHI, BspMII, EcoRI and HindIII. BamHI, BspMII and HindIII are not unique. The BalI site in the polylinker downstream from the λ PL promoter can be used as a unique expression site since the other BalI site cuts only in dcm-negative strains.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727393.1.
Other name of the plasmid is pST236.
EMBL Accession number: LT727393.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 30/01/1989
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: HindII, PvuI and RsaI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr E. Remaut(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: Remaut et al., Gene 15 (1981), 81-93 [PMID: 6271633]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 M5219
Host reference: Remaut et al., Gene 15 (1981), 81-93 [PMID: 6271633]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 28°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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