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LMBP plasmid details

Last data update: 24 November 2020 04:18 CET

Plasmid name: pPLaR1 (LMBP 269)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: pplar1.gb (View with Genome Compiler)
pplar1.pdf
Sequence
analysis results
Genecorner:

-

Cloned DNA: Phage MS2 polymerase gene
Phage MS2 lysis protein gene
Phage MS2 coat protein gene; last 32 codons
Promoter: Phage λ major leftward promoter (λ PL)
Ribosome
binding site:
Ribosome binding site (RBS) of the phage MS2 polymerase gene
Terminator: -
Selection marker: Kanamycin (kan)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli; use strains with a cI function, cIts for PL controlled expression
Parental clone: pPLa2311; pMS27
Further information: The EcoRI-PstI fragment, containing the MS2 polymerase gene, the lysis protein gene and the carboxy terminal part of the coat protein gene, was derived from pMS27 and cloned into the EcoRI-PstI cleaved pPLa2311, in the sense orientation relative to the λ PL promoter.
The exact length of the dA/dT-tails is not known.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727536.1.
Other name of the plasmid is pSRK2311Pol.
EMBL Accession number: LT727536.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 25/03/1992
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: BanII, EcoRI/PstI, HaeII and HindIII.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr E. Remaut(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: Remaut et al., EMBO J. 1 (1982), 205-209 [PMID: 16453413]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 ΔH1Δtrp
Host reference: Bernard et al., Gene 5 (1979), 59-76 [PMID: 372049]
Related host reference: Remaut et al., Gene 15 (1981), 81-93 [PMID: 6271633]
Cultivation medium: LB-Lennox + kanamycin (50 μg/ml)
Cultivation temperature: 28°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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