GREAT AT SMALL THINGS

0

LMBP plasmid details

Last data update: 24 November 2020 04:18 CET

Plasmid name: pPLaIVNA (LMBP 1418)

Add to cart

New search

Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: pplaivna.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Influenza A virus (A/Victoria/3/1975(H3N2)) neuraminidase cDNA (NA, IVNA)
Promoter: Phage λ major leftward promoter (λ PL)
Ribosome
binding site:
-
Terminator: Phage fd terminator
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Parental clone: pPLa2311FdT1; pIVNA
Further information: The plasmid was constructed as follows: pIVNA was opened in the PvuI site (in the pBR322 sequence, at the 5' site of the IVNA gene) and treated with Bal31 exonuclease; then HindIII linkers were added. After cutting with HindIII, the HindIII flanked IVNA gene was inserted in the unique HindIII site of pPLa2311FdT1.
The orientation of the inserted gene is unknown. The length and the sequence between the start codon of IVNA and the preceding, ligated HindIII site are also unknown.
Other name of the plasmid is pPLaNAV125.
EMBL Accession number: -
Latest sequence update: 19/08/1996
Sequence detail:
Nucleotide sequence at the end of the IVNA gene:

CCT.ATA.TAA.GCTT
Pro Ile  *
         HindIII

*: Termination codon of the IVNA gene.
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by C. Lopez(1) and Prof. Dr W. Min Jou(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061(λ)
Host reference: Mertens et al., Gene 164 (1995), 9-15 [PMID: 7590329]
Related host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 28°C
Biosafety level: L1
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

New search