Last data update: 24 November 2020 04:18 CET
Plasmid name: pPLaIVNA (LMBP 1418)
|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner non-core plasmid|
|Cloned DNA:||Influenza A virus (A/Victoria/3/1975(H3N2)) neuraminidase cDNA (NA, IVNA)|
|Promoter:||Phage λ major leftward promoter (λ PL)|
|Terminator:||Phage fd terminator|
|Selection marker:||Ampicillin (amp)|
|Replicon:||Escherichia coli plasmid pMB1 origin|
|Host range:||Escherichia coli|
|Parental clone:||pPLa2311FdT1; pIVNA|
|Further information:||The plasmid was constructed as follows: pIVNA was opened in the PvuI site (in the pBR322 sequence, at the 5' site of the IVNA gene) and treated with Bal31 exonuclease; then HindIII linkers were added. After cutting with HindIII, the HindIII flanked IVNA gene was inserted in the unique HindIII site of pPLa2311FdT1.
The orientation of the inserted gene is unknown. The length and the sequence between the start codon of IVNA and the preceding, ligated HindIII site are also unknown.
Other name of the plasmid is pPLaNAV125.
|EMBL Accession number:||-|
|Latest sequence update:||19/08/1996|
Nucleotide sequence at the end of the IVNA gene: CCT.ATA.TAA.GCTT Pro Ile * HindIII *: Termination codon of the IVNA gene.
|Authenticity test:||The plasmid still needs to be subjected to the authenticity test.|
|History of deposit:||This plasmid was deposited by C. Lopez(1) and Prof. Dr W. Min Jou(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 MC1061(λ)|
|Host reference:||Mertens et al., Gene 164 (1995), 9-15 [PMID: 7590329]
|Related host reference:||Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
|Cultivation medium:||LB-Lennox + ampicillin (100 μg/ml)|
|Other culture collection numbers:||-|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.