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LMBP plasmid details

Last data update: 24 November 2020 04:18 CET

Plasmid name: pPLaACR26 (LMBP 311)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: p311.gb (View with Genome Compiler)
p311.txt
p311.pdf
Sequence
analysis results
Genecorner:

-

Cloned DNA: Phage MS2 coat protein gene
Phage MS2 polymerase gene
Phage MS2 assembly protein gene
Phage MS2 lysis protein gene
Promoter: Phage λ major leftward promoter (λ PL)
Ribosome
binding site:
Ribosome binding site (RBS) of the phage MS2 coat protein gene
Ribosome binding site (RBS) of the phage MS2 polymerase gene
Ribosome binding site (RBS) of the phage MS2 assembly protein gene
Terminator: -
Selection marker: Kanamycin (kan)
Replicon: Escherichia coli plasmid ColE1 origin
Host range: Escherichia coli; use strains with a cI function, cIts for PL controlled expression
Parental clone: pPLa2311; pMS27
Further information: This plasmid contains the coding information of phage MS2, in a sense orientation relative to the λ PL promoter.
The exact length of the dA/dT-tails is not known.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727439.1.
Other name of the plasmid is pSRKMS26.
EMBL Accession number: LT727439.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 25/03/1992
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: BanII, EcoRI/PstI, HindIII and XhoI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr E. Remaut(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: Remaut et al., EMBO J. 1 (1982), 205-209 [PMID: 16453413]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 ΔH1Δtrp
Host reference: Bernard et al., Gene 5 (1979), 59-76 [PMID: 372049]
Related host reference: Remaut et al., Gene 15 (1981), 81-93 [PMID: 6271633]
Cultivation medium: LB-Lennox + kanamycin (50 μg/ml)
Cultivation temperature: 28°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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