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LMBP plasmid details

Last data update: 24 November 2020 04:18 CET

Plasmid name: pPLaA17 (LMBP 295)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: p295.gb (View with Genome Compiler)
p295.txt
p295.pdf
Sequence
analysis results
Genecorner:

-

Cloned DNA: Phage MS2 assembly protein gene
Phage MS2 coat protein gene; fragment
Promoter: Phage λ major leftward promoter (λ PL)
Ribosome
binding site:
Ribosome binding site (RBS) of the phage MS2 assembly protein gene
Ribosome binding site (RBS) of the phage MS2 coat protein gene
Terminator: -
Selection marker: Ampicillin (amp)
Kanamycin (kan)
Replicon: Escherichia coli plasmid ColE1 origin
Host range: Escherichia coli; use strains with a cI function, cIts for PL controlled expression
Parental clone: pPLa2311; pMS27
Further information: The EcoRI fragment of pMS27, containing the complete MS2 assembly protein coding sequence and the coding information for the first 97 amino acids of the MS2 coat protein, was cloned into the EcoRI site of pPLa2311 in the antisense orientation relative to the λ PL promoter.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727559.1.
Other name of the plasmid is pSRKM17.
EMBL Accession number: LT727559.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 25/03/1992
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: AvaI, EcoRI, HindIII and PvuI/StyI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr E. Remaut(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: Remaut et al., EMBO J. 1 (1982), 205-209 [PMID: 16453413]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 M5219
Host reference: Remaut et al., Gene 15 (1981), 81-93 [PMID: 6271633]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml) + kanamycin (50 μg/ml)
Cultivation temperature: 28°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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