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LMBP plasmid details

Last data update: 24 November 2020 04:18 CET

Plasmid name: pPLa832 (LMBP 341)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p341.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: -
Promoter: Phage λ major leftward promoter (λ PL)
Ribosome
binding site:
-
Terminator: -
Selection marker: Kanamycin (kan)
Replicon: Escherichia coli plasmid ColE1 origin
Host range: Escherichia coli; use strains with a cI function, cIts for PL controlled expression
Parental clone: pPLa831
Further information: As compared to pPLa2311, this plasmid provides no resistance to ampicillin.
This plasmid was designed for expression of fragments containing a ribosome binding site functional in E. coli.
Expression sites: BaII, BamHI, EcoRI, FspI and HindIII. There are two HindIII and two BamHI sites in the polylinker downstream from the λ PL promoter. A third HindIII site is located in the kanamycin resistance gene.
Other name of the plasmid is pHS32.
EMBL Accession number: -
Latest sequence update: 29/03/1989
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: HaeII, PvuI and RsaI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr E. Remaut(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: Remaut et al., Gene 15 (1981), 81-93 [PMID: 6271633]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 M5219
Host reference: Remaut et al., Gene 15 (1981), 81-93 [PMID: 6271633]
Cultivation medium: LB-Lennox + kanamycin (50 μg/ml)
Cultivation temperature: 28°C
Biosafety level: L1
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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