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LMBP plasmid details

Last data update: 24 November 2020 04:18 CET

Plasmid name: pPLa8 (LMBP 338)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: ppla8.gb (View with Genome Compiler)
ppla8.pdf
Sequence
analysis results
Genecorner:

-

Cloned DNA: -
Promoter: Phage λ major leftward promoter (λ PL)
Ribosome
binding site:
-
Terminator: -
Selection marker: Kanamycin (kan)
Replicon: Escherichia coli plasmid ColE1 origin
Host range: Escherichia coli; use strains with a cI function, cIts for PL controlled expression
Parental clone: pPLa2311
Further information: The plasmid was constructed by inserting a BamHI linker (5'-CCGGATCCGG-3') into the PstI site located in the ampicillin resistance gene of pPLa2311, which was made blunt by S1 nuclease. This resulted in an inactive β- lactamase, although the reading frame of the ampicillin resistance gene was maintained.
This plasmid was designed for λ PL promoter-regulated expression of fragments containing a ribosome binding site functional in E. coli.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727474.1.
Other name of the plasmid is pSRKB8.
EMBL Accession number: LT727474.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 22/03/1990
Sequence detail:
Nucleotide sequence around the BamHI site in the amp gene:

5' ACC.ACG.ATC.CGG.ATC.CGG.ATG.GCA 3'
                BamHI

Punctuation indicates reading frame in the inactive ß-lactamase.
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: HaeII, HindIII and SspI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr E. Remaut(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: Remaut et al., Gene 15 (1981), 81-93 [PMID: 6271633]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 ΔH1Δtrp
Host reference: Bernard et al., Gene 5 (1979), 59-76 [PMID: 372049]
Related host reference: Remaut et al., Gene 15 (1981), 81-93 [PMID: 6271633]
Cultivation medium: LB-Lennox + kanamycin (50 μg/ml)
Cultivation temperature: 28°C
Biosafety level: L1
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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