Last data update: 24 November 2020 04:18 CET
Plasmid name: pPLa8 (LMBP 338)
|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner core plasmid|
(View with Genome Compiler)
|Promoter:||Phage λ major leftward promoter (λ PL)|
|Selection marker:||Kanamycin (kan)|
|Replicon:||Escherichia coli plasmid ColE1 origin|
|Host range:||Escherichia coli; use strains with a cI function, cIts for PL controlled expression|
|Further information:||The plasmid was constructed by inserting a BamHI linker (5'-CCGGATCCGG-3') into the PstI site located in the ampicillin resistance gene of pPLa2311, which was made blunt by S1 nuclease. This resulted in an inactive β- lactamase, although the reading frame of the ampicillin resistance gene was maintained.
This plasmid was designed for λ PL promoter-regulated expression of fragments containing a ribosome binding site functional in E. coli.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727474.1.
Other name of the plasmid is pSRKB8.
|EMBL Accession number:||LT727474.1, view at EMBL, GenBank, DDBJ|
|Latest sequence update:||22/03/1990|
Nucleotide sequence around the BamHI site in the amp gene: 5' ACC.ACG.ATC.CGG.ATC.CGG.ATG.GCA 3' BamHI Punctuation indicates reading frame in the inactive ß-lactamase.
|Authenticity test:||Restriction enzyme pattern analysed at GeneCorner: HaeII, HindIII and SspI.|
|History of deposit:||This plasmid was deposited by Prof. Dr E. Remaut(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
|Plasmid reference:||Remaut et al., Gene 15 (1981), 81-93 [PMID: 6271633]
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 ΔH1Δtrp|
|Host reference:||Bernard et al., Gene 5 (1979), 59-76 [PMID: 372049]
|Related host reference:||Remaut et al., Gene 15 (1981), 81-93 [PMID: 6271633]
|Cultivation medium:||LB-Lennox + kanamycin (50 μg/ml)|
|Other culture collection numbers:||-|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.