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LMBP plasmid details

Last data update: 24 November 2020 04:18 CET

Plasmid name: pPLa2311trpA1 (LMBP 271)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: p271.gb (View with Genome Compiler)
p271.pdf
Sequence
analysis results
Genecorner:

-

Cloned DNA: Salmonella enterica serovar Typhimurium tryptophan synthetase A gene (trpA)
Promoter: Phage λ major leftward promoter (λ PL)
Ribosome
binding site:
-
Terminator: -
Selection marker: Ampicillin (amp)
Kanamycin (kan)
Replicon: Escherichia coli plasmid ColE1 origin
Host range: Escherichia coli; use strains with a cI function, cIts for PL controlled expression
Parental clone: pPLa2311
Further information: The plasmid was constructed by inserting the 5342 bp large EcoRI fragment from pES9 (containing the Salmonella typhimurium tryptophan synthetase A gene (trpA)) into the EcoRI site of pPLa2311.
The fragment also contains the larger part of the trpB gene, except for the translation initiation region.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727539.1.
The nucleotide sequence of the S. typhimurium fragment corresponds with the Genbank accession number NC_003197.1.
Other name of the plasmid is pSRK2311A1.
EMBL Accession number: NC_003197.1, view at EMBL, GenBank, DDBJ
LT727539.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 06/01/2003
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: EcoRI, HindIII and SalI/XhoI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr E. Remaut(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: Remaut et al., Gene 15 (1981), 81-93 [PMID: 6271633]
Related plasmid reference: Nichols et al., J. Mol. Biol. 142 (1980), 503-517 [PMID: 7007652]
McClelland et al., Nature 413 (2001), 852-856 [PMID: 11677609]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 ΔH1Δtrp
Host reference: Bernard et al., Gene 5 (1979), 59-76 [PMID: 372049]
Related host reference: Remaut et al., Gene 15 (1981), 81-93 [PMID: 6271633]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml) + kanamycin (50 μg/ml)
Cultivation temperature: 28°C
Biosafety level: L1
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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