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LMBP plasmid details

Last data update: 24 November 2020 04:18 CET

Plasmid name: pPLa2311FdT1 (LMBP 321)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: p321.gb (View with Genome Compiler)
p321.pdf
Sequence
analysis results
Genecorner:

-

Cloned DNA: -
Promoter: Phage λ major leftward promoter (λ PL)
Ribosome
binding site:
-
Terminator: Phage fd terminator
Selection marker: Ampicillin (amp)
Kanamycin (kan)
Replicon: Escherichia coli plasmid ColE1 origin
Host range: Escherichia coli; use strains with a cI function, cIts for PL controlled expression
Parental clone: pPLa2311
Further information: pPLa2311FdT1 contains the fd terminator (fdT) and the lac operator fragment (lacO) in the sense orientation relative to the λ PL promoter.
Because of the presence of the lac operator, lac+ E. coli host cells turn blue on X-Gal.
Use the EcoRI site just after the λ PL promoter for expression; the second EcoRI site (after 'fdT-lacO') can't be used for expression.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727451.1.
Other name of the plasmid is pD4.
EMBL Accession number: LT727451.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 27/04/1990
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: HaeII, HindIII and RsaI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr E. Remaut(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: Marmenout et al., Mol. Gen. Genet. 195 (1984), 126-133 [PMID: 6092841]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 ΔH1ΔtrpF'Lac
Host reference: -
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml) + kanamycin (50 μg/ml)
Cultivation temperature: 28°C
Biosafety level: L1
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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