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LMBP plasmid details

Last data update: 24 November 2020 04:18 CET

Plasmid name: pPLa231 (LMBP 217)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: ppla231.gb (View with Genome Compiler)
ppla231.pdf
Sequence
analysis results
Genecorner:

-

Cloned DNA: -
Promoter: Phage λ major leftward promoter (λ PL)
Ribosome
binding site:
-
Terminator: -
Selection marker: Ampicillin (amp)
Kanamycin (kan)
Replicon: Escherichia coli plasmid ColE1 origin
Host range: Escherichia coli; use strains with a cI function, cIts for PL controlled expression
Parental clone: pPLa23; pMK20
Further information: The plasmid is designed for expression of fragments containing a ribosome binding site functional in E. coli.
The plasmid differs from pPLa2311 in the orientation of the kanamycin resistance gene, which is here anticlockwise.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727627.1.
EMBL Accession number: LT727627.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 23/03/1992
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: HindIII, PvuI and RsaI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr E. Remaut(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Related plasmid reference: Remaut et al., Gene 15 (1981), 81-93 [PMID: 6271633]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 M5219
Host reference: Remaut et al., Gene 15 (1981), 81-93 [PMID: 6271633]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml) + kanamycin (50 μg/ml)
Cultivation temperature: 28°C
Biosafety level: L1
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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