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LMBP plasmid details

Last data update: 24 November 2020 04:18 CET

Plasmid name: pPLa10 (LMBP 906)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: ppla10.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: -
Promoter: Phage λ major leftward promoter (λ PL)
Ribosome
binding site:
-
Terminator: -
Selection marker: Ampicillin (amp)
Kanamycin (kan)
Replicon: Escherichia coli plasmid ColE1 origin
Host range: Escherichia coli; use strains with a cI function, cIts for PL controlled expression
Parental clone: pPLa2311
Further information: The PstI site in the ampicillin resistance gene of pPLa2311 was replaced by a SmaI site, resulting in a modified but active β-lactamase.
There is another SmaI site in the kanamycin resistance gene.
EMBL Accession number: -
Latest sequence update: 06/07/1987
Sequence detail:
Nucleotide sequence around the SmaI site in the ampicillin gene:

5' ACC.ACG.ATG.CCC.CGG.GCA.ATG 3'
                SmaI

Punctuation indicates reading frame in the modified active beta-lactamase.
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr E. Remaut(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 K514(λ)
Host reference: Zabeau et al., EMBO J. 1 (1982), 1217-1224 [PMID: 6327257]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml) + kanamycin (50 μg/ml)
Cultivation temperature: 28°C
Biosafety level: L1
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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